Pang Xue, Wan Wei, Wu Xingxing, Shen Yu
Department of Anesthesiology, The Second People's Hospital of Lianyungang, Lianyungang, China.
Department of Hepatobiliary Surgery, The Second People's Hospital of Lianyungang, Lianyungang, China.
Int J Genomics. 2022 Dec 13;2022:8755263. doi: 10.1155/2022/8755263. eCollection 2022.
Hepatocellular carcinoma (HCC) is a common human malignancy with high mortality and dismal prognosis. A growing number of novel targets underlying HCC pathophysiology have been detected using microarray high throughput screening platforms. This study carried out bioinformatics analysis to explore underlying biomarkers in HCC and assessed the potential action of the miR-193b-3p/CDK1 signaling pathway in HCC progression. A total of 241 common differentially expressed genes (DEGs) were screened from GSE33294, GSE104310, and GSE144269. Functional analysis results implicated that DEGs are significantly associated with "cell cycle," "cell division," and "proliferation." The protein-protein interaction network analysis extracted ten hub genes from common DEGs. Ten hub genes were significantly overexpression in HCC tissues. Kaplan-Meier survival analysis revealed that 10 hub genes were linked with a poorer prognosis in HCC patients. Functional assays showed that CDK1 knockdown repressed HCC cell proliferation and migration. Luciferase reporter assay showed that miR-193b-3p could target CDK1 3' untranslated region, and miR-193b-3p negatively modulated CDK1. Enforced CDK1 expression attenuated miR-193b-3p-modulated suppressive actions on HCC cell proliferation and migration. To summarize, we performed a comprehensive bioinformatics analysis and identified 10 hub genes linked to the prognosis in HCC patients. Functional analysis revealed that CDK1, negatively regulated by miR-193b-3p, may act as an oncogene to promote HCC cell proliferation and migration and may predict poor prognosis of HCC patients. However, the role of CDK1/miR-193b-3p may still require further investigation.
肝细胞癌（HCC）是一种常见的人类恶性肿瘤，死亡率高且预后不佳。使用微阵列高通量筛选平台已检测到越来越多与HCC病理生理学相关的新靶点。本研究进行了生物信息学分析，以探索HCC中的潜在生物标志物，并评估miR-193b-3p/CDK1信号通路在HCC进展中的潜在作用。从GSE33294、GSE104310和GSE144269中总共筛选出241个常见的差异表达基因（DEG）。功能分析结果表明，DEG与“细胞周期”、“细胞分裂”和“增殖”显著相关。蛋白质-蛋白质相互作用网络分析从常见的DEG中提取了10个枢纽基因。10个枢纽基因在HCC组织中显著过表达。Kaplan-Meier生存分析显示，10个枢纽基因与HCC患者较差的预后相关。功能试验表明，CDK1敲低可抑制HCC细胞的增殖和迁移。荧光素酶报告基因试验表明，miR-193b-3p可靶向CDK1的3'非翻译区，且miR-193b-3p对CDK1具有负调控作用。强制表达CDK1可减弱miR-193b-3p对HCC细胞增殖和迁移的抑制作用。总之，我们进行了全面的生物信息学分析，确定了10个与HCC患者预后相关的枢纽基因。功能分析表明，受miR-193b-3p负调控的CDK1可能作为癌基因促进HCC细胞增殖和迁移，并可能预测HCC患者的不良预后。然而，CDK1/miR-193b-3p的作用仍可能需要进一步研究。
