Stark O, Kunz H W, Gill T J
J Immunogenet. 1978 Aug;5(4):261-73. doi: 10.1111/j.1744-313x.1978.tb00654.x.
Two haplotypes which posed difficult problems in serological identification, those of the HW and MNR/N strains, were studied. The HW strain was originally described as a unique haplotype (H-1h), but breeding difficulties precluded its detailed serological analysis. The red blood cells of the HW strain agglutinate weakly and cross-react with antisera to the Ag-B8 group. Anti-HW antisera cross-react strongly with LEW, ACI and WKA, but absorption with these strains did not produce an adequate typing serum. By judicious selection of recipients, however, an appropriate typing reagent could be made; a particularly useful one was (BUF X MR)F1 anti-HW absorbed with WKA red blood cells. The HW haplotype segregated appropriately in a (DA X HW)F2 population. The HW strain is a low responder to poly(Glu52Lys33Tyr15). The H-1h haplotype of this strain was designated Ag-B12. The MNR/N strain had not previously been studied serologically, although its MLR type had been defined as H-1c (MLR-5). Antisera to MNR/N cross-reacted strongly with the H-1a,b,d,f haplotypes, but MNR/N red blood cells agglutinated only weakly with many antisera. An operationally monospecific reagent antiserum to the MNR/N haplotype could not be made. The uniqueness of the MNR/N haplotype was shown by F1 tests with LEW.1A, LEW.1D and LEW.1F, by various serological analyses, including production of antisera against MNR/N and in the MR strain; by segregation studies with (LEW X LEW.1D)N5 and (DA X DA.MNR)N4 segregating back-cross populations, and by grafting skin from (DA X DA.MNR)N4 homozygous and heterozygous animals to DA recipients. The MNR/N strain is a high-responder to poly(Glu52Lys33Tyr15). The MNR/N haplotype of this strain was designated Ag-B13 (H-1m). The data led to the working hypothesis that the MNR/N strain may be a recombination between the A region of H-1d and the B region of H-1c. In addition, the H-1d private specificity at the A region was probably lost by a deletion mutation which left the main complex of public specificities intact.
研究了在血清学鉴定中存在难题的两种单倍型，即HW和MNR/N品系的单倍型。HW品系最初被描述为一种独特的单倍型（H-1h），但繁殖困难妨碍了对其进行详细的血清学分析。HW品系的红细胞凝集较弱，并与针对Ag-B8组的抗血清发生交叉反应。抗HW抗血清与LEW、ACI和WKA强烈交叉反应，但用这些品系吸收后并不能产生合适的分型血清。然而，通过明智地选择受体，可以制备出合适的分型试剂；一种特别有用的试剂是用WKA红细胞吸收的（BUF×MR）F1抗HW。HW单倍型在（DA×HW）F2群体中正确分离。HW品系对聚（Glu52Lys33Tyr15）反应较弱。该品系的H-1h单倍型被指定为Ag-B12。MNR/N品系以前没有进行过血清学研究，尽管其混合淋巴细胞反应（MLR）类型已被定义为H-1c（MLR-5）。针对MNR/N的抗血清与H-1a、b、d、f单倍型强烈交叉反应，但MNR/N红细胞与许多抗血清的凝集反应较弱。无法制备针对MNR/N单倍型的操作上单一特异性的试剂抗血清。通过与LEW.1A、LEW.1D和LEW.1F进行F1试验、各种血清学分析（包括制备针对MNR/N和MR品系的抗血清）、用（LEW×LEW.1D）N5和（DA×DA.MNR）N4分离回交群体进行分离研究以及将（DA×DA.MNR）N4纯合和杂合动物的皮肤移植到DA受体上，证明了MNR/N单倍型的独特性。MNR/N品系对聚（Glu52Lys33Tyr15）反应较强。该品系的MNR/N单倍型被指定为Ag-B13（H-1m）。这些数据得出一个工作假设，即MNR/N品系可能是H-1d的A区域与H-1c的B区域之间的重组产物。此外，A区域的H-1d私有特异性可能因缺失突变而丢失，而公共特异性的主要复合体保持完整。
