Radka S F, Cramer D V, Gill T J
J Immunol. 1977 Dec;119(6):2037-44.
Three different alloantisera were raised by using Ag-B/MLR disparate rats, and the cytotoxic activity remaining after absorption with erythrocytes to remove anti-Ag-B antibodies was examined. The alloantisera detected surface antigens present only on B cells and segregation studies demonstrated that the genes that code for these antigenic specificities were linked to the major histocompatibility complex. The reactivity of the alloantisera with splenic lymphocytes from a panel of strains representative of the currently known Ag-B groups showed that multiple specificities were present in two of the three antisera and that these specificities were shared by many inbred strains. The appropriate absorption studies showed, however, that each antiserum detected an unique specificity that was found only in those inbred strains that shared the same mixed lymphocyte reactivity (MLR) phenotype as the donor strain. The alloantiserum produced against the KGH strain inhibited the MLR reactions involving this strain only when it was used as the stimulating cell population. The antigens detected by the three alloantisera described here have the characteristics of Ia antigens, and they have tentatively been designated Ia.1 (ACI anti-KGH), Ia.3 (B3 anti-BN) and Ia.4 (MNR anti-DA).
通过使用Ag - B/混合淋巴细胞反应（MLR）不相容的大鼠制备了三种不同的同种抗血清，并检测了用红细胞吸收以去除抗Ag - B抗体后剩余的细胞毒性活性。这些同种抗血清检测到仅存在于B细胞表面的抗原，分离研究表明编码这些抗原特异性的基因与主要组织相容性复合体相连。用一组代表目前已知Ag - B组的品系的脾淋巴细胞检测同种抗血清的反应性，结果显示三种抗血清中的两种存在多种特异性，并且这些特异性为许多近交系所共有。然而，适当的吸收研究表明，每种抗血清检测到一种独特的特异性，这种特异性仅在那些与供体品系具有相同混合淋巴细胞反应（MLR）表型的近交系中发现。针对KGH品系产生的同种抗血清仅在用作刺激细胞群体时才抑制涉及该品系的MLR反应。这里描述的三种同种抗血清检测到的抗原具有Ia抗原的特征，它们被暂时命名为Ia.1（ACI抗KGH）、Ia.3（B3抗BN）和Ia.4（MNR抗DA）。
