基于网络药理学和实验验证的芍药甘草汤治疗胃癌机制研究。

Investigation on the mechanism of Shaoyao-Gancao Decoction in the treatment of gastric carcinoma based on network pharmacology and experimental verification.

机构信息

Traditional Chinese Medicine College, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous, China.

Key Laboratory of Ningxia Minority Medicine Modernization Ministry of Education, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China.

出版信息

Aging (Albany NY). 2023 Jan 3;15(1):148-163. doi: 10.18632/aging.204465.

BACKGROUND

Shaoyao-Gancao Decoction (SG-D) is a famous classical Chinese prescription that has been used in the treatment of numerous kinds of diseases. However, its mechanism of action in the treatment of Gastric carcinoma (GC) is not clear.

METHODS

The active ingredients and targets of SG-D were screened using network pharmacology, and GC-related targets were retrieved through several databases. The protein-protein interaction network was then further constructed and GO and KEGG enrichment analysis were performed. Subsequently, molecular docking was carried out. Finally, we validated the results of the network pharmacology by performing cell experiments on CCK-8, apoptosis, cell cycle, platelet clone formation, and Western blotting with AGS cells.

RESULTS

Three key active ingredients and 8 core targets were screened through a network pharmacological analysis, and the results of the KEGG indicated that the PI3K/Akt and MAPK signaling pathways are critical signaling pathways for SG-D to treat GC. Experimental results revealed that SG-D was able to inhibit AGS cells proliferation, induce apoptosis and arrest the cell cycle, and reduce the ability of cell clone formation by regulating the PI3K/Akt and MAPK signaling pathways.

CONCLUSIONS

Network pharmacology has shown that SG-D can act on multiple targets through multiple ingredients and treat GC by regulating multiple signaling pathways. cell experiments have also confirmed this, so as to provide a reference for subsequent related research.

背景

芍药甘草汤（SG-D）是一种著名的经典中药方剂，已被用于治疗多种疾病。然而，其在胃癌（GC）治疗中的作用机制尚不清楚。

方法

采用网络药理学筛选 SG-D 的活性成分和靶点，并通过多个数据库检索 GC 相关靶点。进一步构建蛋白质-蛋白质相互作用网络，并进行 GO 和 KEGG 富集分析。然后进行分子对接。最后，通过 CCK-8、细胞凋亡、细胞周期、血小板克隆形成和 Western blot 实验，对 AGS 细胞进行细胞实验，验证网络药理学的结果。

结果

通过网络药理学分析筛选出 3 个关键活性成分和 8 个核心靶点，KEGG 结果表明，PI3K/Akt 和 MAPK 信号通路是 SG-D 治疗 GC 的关键信号通路。实验结果表明，SG-D 能够通过调节 PI3K/Akt 和 MAPK 信号通路抑制 AGS 细胞增殖，诱导细胞凋亡和细胞周期阻滞，降低细胞克隆形成能力。