Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea; Institute for Ubiquitous Information Technology and Application, Konkuk University, Seoul 05029, Republic of Korea.
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea.
Bioresour Technol. 2023 Feb;370:128571. doi: 10.1016/j.biortech.2022.128571. Epub 2023 Jan 2.
In this study, fourteen types of biochar produced using seven biomasses at temperatures 300 °C and 600 °C were screened for phenolics (furfural and hydroxymethylfurfural (HMF)) removal. Eucheuma spinosum biochar (EB-BC 600) showed higher adsorption capacity to furfural (258.94 ± 3.2 mg/g) and HMF (222.81 ± 2.3 mg/g). Adsorption kinetics and isotherm experiments interpreted that EB-BC 600 biochar followed the pseudo-first-order kinetic and Langmuir isotherm model for both furfural and HMF adsorption. Different hydrolysates were detoxified using EB-BC 600 biochar and used as feedstock for engineered Escherichia coli. An increased polyhydroxyalkanoates (PHA) production with detoxified barley biomass hydrolysate (DBBH: 1.71 ± 0.07 g PHA/L), detoxified miscanthus biomass hydrolysate (DMBH: 0.87 ± 0.03 g PHA/L) and detoxified pine biomass hydrolysate (DPBH: 1.28 ± 0.03 g PHA/L) was recorded, which was 2.8, 6.4 and 3.4 folds high as compared to undetoxified hydrolysates. This study reports the mechanism involved in furfural and HMF removal using biochar and valorization of hydrolysate into PHA.
在这项研究中，筛选了七种生物质在 300°C 和 600°C 下生产的 14 种生物炭，以去除酚类物质（糠醛和羟甲基糠醛（HMF））。螺旋藻生物炭（EB-BC 600）对糠醛（258.94±3.2mg/g）和 HMF（222.81±2.3mg/g）具有更高的吸附能力。吸附动力学和等温线实验表明，EB-BC 600 生物炭遵循伪一级动力学和 Langmuir 等温线模型，用于糠醛和 HMF 的吸附。使用 EB-BC 600 生物炭对不同水解物进行解毒，并将其用作工程大肠杆菌的原料。经解毒大麦生物质水解物（DBBH：1.71±0.07gPHA/L）、经解毒芒草生物质水解物（DMBH：0.87±0.03gPHA/L）和经解毒松木生物质水解物（DPBH：1.28±0.03gPHA/L）的聚羟基烷酸（PHA）产量增加，分别比未解毒水解物高 2.8、6.4 和 3.4 倍。本研究报告了生物炭去除糠醛和 HMF 的机制以及水解物转化为 PHA 的增值。
