Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea; Institute for Ubiquitous Information Technology and Applications, Seoul 05029, Republic of Korea.
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea.
Bioresour Technol. 2022 Oct;361:127753. doi: 10.1016/j.biortech.2022.127753. Epub 2022 Aug 6.
Sphingobium yanoikuyae BBL01 can produce exopolysaccharides (EPS) and polyhydroxyalkanoates (PHAs). The effect of side products (furfural, hydroxymethylfurfural (HMF), vanillin, and acetate) produced during pretreatment of biomass was evaluated on S. yanoikuyae BBL01. It was observed that a certain concentration range (0.01-0.03 %) of these compounds can improve growth, EPS production, and polyhydroxybutyrate (PHB) accumulation. The addition of HMF increases glucose and xylose utilization while other side products have a negative effect. The C/N of 5 favors EPS production (3.24 ± 0.05 g/L), while a higher C/N ratio of 30 promotes PHB accumulation (38.7 ± 0.08 % w/w), when commercial sugar is used as a carbon source. Pine biomass-derived biochar was able to remove 40 ± 2.1 % of total phenolic. Various biomass hydrolysates were evaluated and the use of detoxified pine biomass hydrolysate (DPH) as a carbon source resulted in the higher coproduction of EPS (2.83 ± 0.03 g/L) and PHB (40.8 ± 2.4 % w/w).
黄杆菌（Sphingobium yanoikuyae）BBL01 可以产生胞外多糖（EPS）和聚羟基脂肪酸酯（PHA）。评估了生物量预处理过程中产生的副产物（糠醛、羟甲基糠醛（HMF）、香草醛和乙酸盐）对 S. yanoikuyae BBL01 的影响。结果表明，这些化合物在一定浓度范围内（0.01-0.03%）可以促进生长、EPS 生产和聚羟基丁酸酯（PHB）积累。HMF 的添加增加了葡萄糖和木糖的利用率，而其他副产物则有负面影响。当使用商业糖作为碳源时，C/N 为 5 有利于 EPS 生产（3.24±0.05g/L），而 C/N 比为 30 时有利于 PHB 积累（38.7±0.08%w/w）。松树生物质衍生的生物炭能够去除总酚的 40±2.1%。评估了各种生物质水解物，使用解毒松树生物质水解物（DPH）作为碳源可导致 EPS（2.83±0.03g/L）和 PHB（40.8±2.4%w/w）的更高共产量。
