Water Treatment and Recycling Laboratory, Water Research and Technology Center (CERTE), Technopark Borj-Cédria, Soliman, Tunisia.
Eremology and Combating Desertification (LR16IRA01) Arid Regions Institute of Medenine, Medenine, Tunisia.
Int J Phytoremediation. 2023;25(11):1474-1487. doi: 10.1080/15226514.2022.2164249. Epub 2023 Jan 6.
The aim of this study was the comparison of two process in pentachlorophenol (PCP: 100 mg L) removal by combined process bioaugmentation-adsorption and bioaugmentation-phytoremediation in secondary treated wastewater (STWW). The phytoremediation procedure was conducted by using two plants such as and , and the bioaugmentation procedure was operated by HM 627618 as a plant growth promoting bacteria (PGPR). The adsorption process was performed by palm date activated carbon. The PCP monitoring was assessed by high performance liquid chromatography (HPLC) and the optical density determination at 600 nm (OD). The performance of the two processes was observed by the determination of total bacteria, chlorophylls and physical and chemical analysis (COD, pH, conductivity, chloride, and organic carbon). The alfalfa seed germination test was conducted to assess the two operational performance procedures. According to the results obtained from the physical and chemical analysis of the treated STWW, there was no significant differences in the pH and in the EC content of the bioaugmentation-phytoremediation treatment, while a significant increase of the EC content was observed in the bioaugmentation-adsorption to 5.08 mS cm. The COD value significantly decreased up to 1320 mg L in bioaugmentation-adsorption treatment (control value 2400 mg L) and 98 mg L in bioaugmentation-phytoremediation treatment (control value 98 mg L). Microbial biomass monitoring of shows significant greater in both processes in the order of 9.18 and 7.01 Log CFU mL for bioaugmentation-adsorption and bioaugmentation-phytoremediation, respectively. The chlorophyll content in and significantly decreased after 144 h with the exception of the chlorophyll a content of in which the content increased up to 3.31 mg mL. Comparing the performance of these two treatments, it was found according to HPLC analysis that the bioaugmentation-adsorption process was more efficient in removing about 97% of PCP after 48 h, against around 90% of PCP after 72 h for the phytoremediation-bioaugmentation. The alfalfa seeds showed a germination rate after the 5th day of incubation of 100% and 95%, respectively for the PCP-non-contaminated and treated STWW, while for wastewater containing PCP the germination was totally inhibited.
本研究旨在比较组合工艺生物强化-吸附和生物强化-植物修复在二级处理废水中（STWW）去除五氯苯酚（PCP：100mg/L）的两种工艺。植物修复过程采用 和 两种植物进行，生物强化过程采用 HM 627618 作为植物生长促进细菌（PGPR）进行。吸附过程采用棕榈核活性炭进行。通过高效液相色谱法（HPLC）和 600nm 处的光密度测定（OD）评估 PCP 监测。通过测定总细菌、叶绿素和物理化学分析（COD、pH、电导率、氯化物和有机碳）来观察两种工艺的性能。进行了紫花苜蓿种子发芽试验，以评估两种操作性能程序。根据处理后的 STWW 的物理化学分析结果，生物强化-植物修复处理的 pH 和 EC 含量没有显著差异，而生物强化-吸附处理的 EC 含量显著增加到 5.08mS/cm。COD 值在生物强化-吸附处理中显著降低至 1320mg/L（对照值为 2400mg/L），在生物强化-植物修复处理中降低至 98mg/L（对照值为 98mg/L）。两种工艺中 的微生物生物量监测结果均显示，生物强化-吸附和生物强化-植物修复的顺序分别为 9.18 和 7.01 Log CFU/mL。144 小时后， 和 中的叶绿素含量显著下降，但 中的叶绿素 a 含量增加到 3.31mg/mL。比较这两种处理方法的性能，根据 HPLC 分析发现，生物强化-吸附工艺在 48 小时后去除 PCP 的效率更高，约为 97%，而生物强化-植物修复工艺在 72 小时后去除 PCP 的效率约为 90%。紫花苜蓿种子在孵育第 5 天的发芽率分别为 100%和 95%，分别用于未污染和处理后的 STWW，而含有 PCP 的废水则完全抑制了发芽。
