Laboratoire de Traitement Et Valorisation Des Rejets Hydriques (LTVRH), Water Research and Technology Center (CERTE), Technopole Borj-Cédria, B.P. 273, 8020, Soliman, Tunisia.
Eremology and Combating Desertification, Arid Regions Institute of Medenine, Medenine, Tunisia.
Arch Microbiol. 2021 Dec;203(10):6231-6243. doi: 10.1007/s00203-021-02589-9. Epub 2021 Sep 30.
The aims of this study were (i) to compare PCP removal (100 mg L) by two bacterial consortia B and B in sterile wastewater (STWW) and liquid mineral medium (MSM), (ii) PCP effect in biofilm formation and antimicrobial susceptibility. PCP removal was measured by high-performance liquid chromatography (HPLC) during 168 h at 30 °C. Biofilm formation was assessed with two approaches: Congo Red Agar and Microtiter-plate. Antimicrobial susceptibility was determined by the agar disc diffusion technique. The results showed that the PCP removal for consortium B and B after 168 h was 70 and 97.5% in STWW; 62.2 and 85.5% in MSM, respectively. In addition, PCP addition showed an increase in biofilm development especially for B consortium around 3.5 nm in 100 mg L PCP. PCP added in the Muller Hinton (MH) medium and Gentamicin disc showed a clear increase in diameter of cell lysis around 2 to 4.5 cm.
(i) 比较两种细菌群落 B 和 B 在无菌废水 (STWW) 和液体矿物培养基 (MSM) 中去除对氯苯氧乙酸 (PCP) 的效果；(ii) 研究 PCP 对生物膜形成和抗菌药物敏感性的影响。在 30°C 下用高效液相色谱法 (HPLC) 测定 168 小时内的 PCP 去除率。采用两种方法评估生物膜形成：刚果红琼脂法和微量滴定板法。采用琼脂扩散法测定抗菌药物敏感性。结果表明，168 小时后，B 和 B 群落分别在 STWW 中去除了 70%和 97.5%的 PCP；在 MSM 中去除了 62.2%和 85.5%的 PCP。此外，PCP 的添加增加了生物膜的发展，特别是在 100mg/L PCP 下 B 群落的生物膜发展增加了约 3.5nm。在 Muller Hinton (MH) 培养基和庆大霉素药敏纸片中添加 PCP 后，细胞裂解直径明显增加了约 2 到 4.5cm。
