Stellenbosch University, Department of Chemistry and Polymer Science, Private Bag X1, Matieland 7602, South Africa.
Medical Biotechnology and Immunotherapy Research Unit, Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory 7935, South Africa.
Biomacromolecules. 2023 Feb 13;24(2):517-530. doi: 10.1021/acs.biomac.2c01238. Epub 2023 Jan 6.
The conjugation of proteins with synthetic molecules can be conducted in many different ways. In this Perspective, we focus on tag-based techniques and specifically on the SNAP-tag technology. The SNAP-tag technology makes use of a fusion protein between a protein of interest and an enzyme tag that enables the actual conjugation reaction. The SNAP-tag is based on the -alkylguanine-DNA alkyltransferase (AGT) enzyme and is optimized to react selectively with -benzylguanine (BG) substrates. BG-containing dye derivatives have frequently been used to introduce a fluorescent tag to a specific protein. We believe that the site-specific conjugation of polymers to proteins can significantly benefit from the SNAP-tag technology. Especially, polymers synthesized via reversible deactivation radical polymerization allow for the facile introduction of a BG end group to enable SNAP-tag conjugation.
蛋白质与合成分子的缀合可以通过多种不同的方式进行。在本观点中，我们专注于基于标记的技术，特别是 SNAP 标签技术。SNAP 标签技术利用融合蛋白，将目标蛋白与酶标签融合，从而实现实际的缀合反应。SNAP 标签基于 -烷基鸟嘌呤-DNA 烷基转移酶（AGT）酶，并经过优化，可选择性地与 -苯甲基鸟嘌呤（BG）底物反应。含有 BG 的染料衍生物经常被用于将荧光标记引入特定的蛋白质。我们相信，聚合物与蛋白质的定点缀合将从 SNAP 标签技术中受益匪浅。特别是，通过可逆失活自由基聚合合成的聚合物可以方便地引入 BG 端基，从而实现 SNAP 标签缀合。
