环状AGK通过miR-3180-5p/BAX轴调节双氢睾酮诱导的毛囊乳头细胞凋亡。
CircAGK regulates high dihydrotestosterone-induced apoptosis in DPCs through the miR-3180-5p/BAX axis.
作者信息
Wei Hanxiao, Yang Shuai, Yi Tian, Xu Xiaoyu, Liu Chang, Shen Caiqi, Guo Yanping, Li Qiang, Jin Peisheng
机构信息
Department of Plastic Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
The First College of Clinical Medicine, Xuzhou Medical University, Xuzhou, China.
出版信息
FASEB J. 2023 Feb;37(2):e22728. doi: 10.1096/fj.202200849R.
The incidence of androgen alopecia (AGA), also known as seborrheic alopecia, has surged in recent years, and onset is occurring at younger ages. Dermal papilla cells (DPCs) are key to maintaining hair cycling, and apoptosis-driven processes in DPCs are closely related to hair follicle regeneration. Circular RNAs (circRNAs) are widely present in the human body and are closely related to the occurrence and development of many diseases. Currently, the biological functions of circRNAs in AGA are largely unknown. Whole-transcriptome sequencing was used to screen differential circRNA expression profiles between AGA patients and non-AGA patients. We found that hsa_circ_0002980 (circAGK) was significantly highly expressed in the AGA group. CircAGK promoted DPC apoptosis in the presence of high dihydrotestosterone (DHT) (15 nmol/L). By regulating the miR-3180-5p/BAX axis, circAGK promotes DPC apoptosis in a high DHT environment in vitro and inhibits hair growth in AGA mice in vivo, indicating that circAGK is a potential target for the clinical treatment of AGA.
雄激素性脱发（AGA），又称脂溢性脱发，近年来发病率激增，且发病年龄趋于年轻化。毛乳头细胞（DPCs）是维持毛发周期的关键，DPCs中由凋亡驱动的过程与毛囊再生密切相关。环状RNA（circRNAs）广泛存在于人体中，与多种疾病的发生发展密切相关。目前，circRNAs在AGA中的生物学功能 largely未知。采用全转录组测序筛选AGA患者和非AGA患者之间的差异circRNA表达谱。我们发现hsa_circ_0002980（circAGK）在AGA组中显著高表达。在高浓度双氢睾酮（DHT）（15 nmol/L）存在的情况下，circAGK促进DPC凋亡。通过调节miR-3180-5p/BAX轴，circAGK在体外高DHT环境中促进DPC凋亡，并在体内抑制AGA小鼠的毛发生长，表明circAGK是AGA临床治疗的潜在靶点。
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