血小板糖蛋白IIIa上Yukb同种抗原的鉴定。
Identification of the Yukb allo-antigen on platelet glycoprotein IIIa.
作者信息
Santoso S, Shibata Y, Kiefel V, Mueller-Eckhardt C
机构信息
Institute of Clinical Immunology and Blood Transfusion, Justus Liebig University, Giessen, FRG.
出版信息
Vox Sang. 1987;53(1):48-51. doi: 10.1111/j.1423-0410.1987.tb04913.x.
The glycoprotein (GP) localization of a new platelet-specific allo-antigen Yukb is described. The antibody was isolated from serum of a patient with neonatal allo-immune thrombocytopenia. In immunoblot procedure, it bound exclusively to platelet GP IIIa, like anti-PlA1, while the known anti-Baka and anti-Leka reacted with GP IIb. Analysis of GP from chymotrypsin-treated platelets with anti-Yukb revealed no binding in the 68-kilodalton position while anti-PlA1 did. Thus, unlike the PlA1 antigen, the Yukb determinant either resides on the 30-kilodalton fragment of GP IIIa or it is destroyed by chymotrypsin treatment.
描述了一种新的血小板特异性同种抗原Yukb的糖蛋白（GP）定位。该抗体从一名新生儿同种免疫性血小板减少症患者的血清中分离得到。在免疫印迹实验中，它与抗PlA1一样，仅与血小板GP IIIa结合，而已知的抗Baka和抗Leka则与GP IIb反应。用抗Yukb分析经胰凝乳蛋白酶处理的血小板中的GP，发现在68千道尔顿位置无结合，而抗PlA1有结合。因此，与PlA1抗原不同，Yukb决定簇要么位于GP IIIa的30千道尔顿片段上，要么被胰凝乳蛋白酶处理破坏。
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