Department of Dermatology, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
Construction Unit of Branch Center of National Clinical Research Center for Dermatologic and Immunological Diseases, Ganzhou, China.
PLoS One. 2023 Jan 6;18(1):e0279908. doi: 10.1371/journal.pone.0279908. eCollection 2023.
Gasdermin (GSDM) B is a member of the GSDM family, which is a protein that may be involved in the cell pyroptosis process and is associated with inflammatory diseases.
To explore the correlation between GSDMB and psoriasis vulgaris.
Skin lesions from 33 patients with psoriasis vulgaris and 69 normal controls were collected. ELISA and Western blot were adopted to detect proteins. The HaCaT cell line was transfected with 3 sets of interfering sequence siRNA, and the mRNA and protein levels before and after the transfection were measured by qPCR and Western blot respectively, so as to establish a cell model with low GSDMB gene expression; the MTT method was used to detect cells viability, flow cytometry to detect cell apoptosis.
The level of GSDMB protein in the skin lesions of patients with psoriasis vulgaris was lower than that in normal skin tissues (P < 0.05). The mRNA and protein expression levels of the target gene in the siRNA-GSDMB-3 group were lower than those in the control group (P < 0.05). The proliferation of HaCaT cells was decreased by MTT method and flow cytometry, and the apoptosis rate was increased (P < 0.05).
The expression level of GSDMB in psoriasis vulgaris lesion tissue is lower than that of normal skin tissue. The down-regulation of GSDMB expression can inhibit cell proliferation and promote cell apoptosis. GSDMB may play a role in the pathogenesis of psoriasis by affecting the differentiation of keratinocytes and the function of T cells.
Gasdermin (GSDM) B 是 GSDM 家族的一员，该家族蛋白可能参与细胞焦亡过程，并与炎症性疾病有关。
探讨 GSDMB 与寻常型银屑病的相关性。
收集 33 例寻常型银屑病患者皮损及 69 例正常人对照皮肤组织，采用 ELISA 及 Western blot 检测蛋白水平。采用 3 组干扰序列 siRNA 转染 HaCaT 细胞系，分别通过 qPCR 和 Western blot 检测转染前后的 mRNA 和蛋白水平，构建 GSDMB 基因低表达的细胞模型；采用 MTT 法检测细胞活力，流式细胞术检测细胞凋亡。
寻常型银屑病患者皮损组织中 GSDMB 蛋白水平低于正常皮肤组织（P < 0.05）。siRNA-GSDMB-3 组的目的基因 mRNA 和蛋白表达水平均低于对照组（P < 0.05）。MTT 法和流式细胞术检测显示，HaCaT 细胞的增殖能力降低，凋亡率增加（P < 0.05）。
寻常型银屑病皮损组织中 GSDMB 的表达水平低于正常皮肤组织。下调 GSDMB 的表达可抑制细胞增殖，促进细胞凋亡。GSDMB 可能通过影响角质形成细胞的分化和 T 细胞的功能参与银屑病的发病机制。
