Jiang Shanshan, Miao Jingjing, Wang Lu, Yao Linlin, Pan Luqing
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
Comp Biochem Physiol A Mol Integr Physiol. 2023 Mar;277:111367. doi: 10.1016/j.cbpa.2022.111367. Epub 2023 Jan 3.
Gonadotropin-releasing hormone (GnRH) plays a key role in the control of the reproductive axis in vertebrates, however, little is known about its function in reproductive endocrine regulation in molluscs. In the present study, RNA-seq was used to construct transcriptomes of Ruditapes philippinarum testis and ovaries of control and GnRH suppressed individuals using RNA interference. GnRH suppression caused 112 and 169 enriched KEGG pathways in testis and ovary, with 92 pathways in common in both comparisons. The most enriched KEGG pathways occurred in the "Oxidative phosphorylation", "Dorso-ventral axis formation", "Thyroid hormone synthesis" and "Oxytocin signaling pathway" etc. A total of 1838 genes in testis and 358 genes in ovaries were detected differentially expressed in GnRH suppressed clams. Among the differentially expressed genes, a suit of genes related to regulation of steroid hormones synthesis and gonadal development, were found in both ovary and testis with RNAi of GnRH. These results suggest that GnRH may play an important role in reproductive function in bivalves. This study provides a preliminary basis for studying the function and regulatory mechanism of GnRH in bivalves.
促性腺激素释放激素（GnRH）在脊椎动物生殖轴的调控中起着关键作用，然而，关于其在软体动物生殖内分泌调节中的功能却知之甚少。在本研究中，利用RNA干扰技术，通过RNA测序构建了对照组以及GnRH抑制组菲律宾蛤仔睾丸和卵巢的转录组。GnRH抑制导致睾丸和卵巢中分别有112条和169条KEGG富集通路，两者共有92条通路。最富集的KEGG通路出现在“氧化磷酸化”“背腹轴形成”“甲状腺激素合成”和“催产素信号通路”等方面。在GnRH抑制的蛤仔中，共检测到睾丸中有1838个基因、卵巢中有358个基因差异表达。在差异表达基因中，通过GnRH的RNA干扰在卵巢和睾丸中均发现了一组与类固醇激素合成调控和性腺发育相关的基因。这些结果表明，GnRH可能在双壳贝类的生殖功能中发挥重要作用。本研究为探讨GnRH在双壳贝类中的功能及调控机制提供了初步依据。
