Zuo Shuqi, Jiang Kunyin, Li Dongdong, Yan Xiwu, Nie Hongtao
Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, Dalian Ocean University, Dalian, 116023, China; College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China.
Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, Dalian Ocean University, Dalian, 116023, China; College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China.
Fish Shellfish Immunol. 2020 Nov;106:110-119. doi: 10.1016/j.fsi.2020.07.022. Epub 2020 Aug 2.
The Manila clam, Ruditapes philippinarum, is an economically important shellfish in marine aquaculture. A better understanding of the immune system in R. philippinarum will provide the basis for the development of strategies to mitigate the impact of infectious diseases affecting this species but can also be of relevance for other bivalves of commercial interest. In this study, the transcriptional response of the Manila clam under lipopolysaccharide (LPS) challenge was characterized using RNA sequencing. The transcriptomes of LPS challenged group of clams (LH1, LH2 and LH3), and the PBS control group (CH1, CH2 and CH3), were sequenced with the Illumina HiSeq platform. Compared with the unigene expression profile of the control group, 223 unigenes were up-regulated and 389 unigenes were down-regulated in the LPS challenged group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that signal transduction, defense response, and immune-related pathways such as Chemokine signaling pathway, Complement and coagulation cascades, NOD-like receptor signaling pathway, and Inflammatory mediator regulation of TRP channels in sensory system were the most highly enriched pathways among the genes that were differentially expressed under LPS challenge. This study present understanding of the molecular basis underpinning response to LPS challenge and provides useful information for future work on the molecular mechanism of pathogen resistance and immunity in Manila clam.
菲律宾蛤仔（Ruditapes philippinarum）是海水养殖中具有重要经济价值的贝类。深入了解菲律宾蛤仔的免疫系统将为制定减轻影响该物种的传染病影响的策略提供依据，同时也可能与其他具有商业价值的双壳贝类相关。在本研究中，利用RNA测序对菲律宾蛤仔在脂多糖（LPS）刺激下的转录反应进行了表征。使用Illumina HiSeq平台对LPS刺激组的蛤仔（LH1、LH2和LH3）以及PBS对照组（CH1、CH2和CH3）的转录组进行了测序。与对照组的单基因表达谱相比，LPS刺激组中有223个单基因上调，389个单基因下调。基因本体（GO）和京都基因与基因组百科全书（KEGG）分析表明，信号转导、防御反应以及免疫相关途径，如趋化因子信号通路、补体和凝血级联反应、NOD样受体信号通路以及感觉系统中TRP通道的炎症介质调节，是LPS刺激下差异表达基因中富集程度最高的途径。本研究揭示了菲律宾蛤仔对LPS刺激反应的分子基础，为未来关于菲律宾蛤仔病原体抗性和免疫分子机制的研究提供了有用信息。
