College of Plant Protection, Shanxi Agricultural University, 030031, Taiyuan, China.
Mol Biol Rep. 2023 Mar;50(3):2501-2510. doi: 10.1007/s11033-022-07869-1. Epub 2023 Jan 6.
Tetanops sintenisi is a pest that mainly damages the root of quinoa (Chenopodium quinoa) and it is first discovered in China in 2018.
Here, the complete mitochondrial genome (mitogenome) of T. sintenisi was sequenced and compared with the mitogenomes of other Diptera species. The results revealed that the mitogenome of T. sintenisi is 15,763 bp in length (GenBank accession number: MT795181) and is comprised of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA genes, and a non-coding A + T-rich region (959 bp). The highly conserved gene arrangement of the mitogenome of T. sintenisi was identical to that of other Diptera insects. Twelve PCGs contained the typical insect start codon ATN, while cox1 had CGA as the start codon. The genes cox2, nad4, and nad1 contained an incomplete termination codon T; nad3, nad5, and cob contained the complete termination codon TAG; and the remaining seven PCGs contained the termination codon TAA. All tRNA genes were predicted to fold into the typical cloverleaf secondary structure. Phylogenetic analysis of 48 species based on the mitogenome sequence revealed that T. sintenisi clustered with the Tephritidae family, indicating that T. sintenisi and Tephritidae have a close phylogenetic relationship.
The phylogenetic relationship of T. sintenisi based on the mitogenome is consistent with the traditional morphological taxonomy, according to which T. sintenisi belongs to the family Otitidae, which is closely related to the family Muscidae.
Tetanops sintenisi 是一种主要损害藜麦（Chenopodium quinoa）根部的害虫，于 2018 年在中国首次发现。
本文对 T. sintenisi 的完整线粒体基因组（mitogenome）进行了测序，并与其他双翅目物种的 mitogenomes 进行了比较。结果表明，T. sintenisi 的 mitogenome 长 15763bp（GenBank 登录号：MT795181），由 13 个蛋白质编码基因（PCGs）、22 个转移 RNA（tRNA）基因、2 个核糖体 RNA 基因和一个非编码 A+T 丰富区（959bp）组成。T. sintenisi mitogenome 的高度保守基因排列与其他双翅目昆虫相同。12 个 PCGs 包含典型的昆虫起始密码子 ATN，而 cox1 以 CGA 作为起始密码子。cox2、nad4 和 nad1 基因含有不完整的终止密码子 T；nad3、nad5 和 cob 含有完整的终止密码子 TAG；其余七个 PCGs 含有终止密码子 TAA。所有 tRNA 基因均预测为折叠成典型的三叶草二级结构。基于 mitogenome 序列对 48 种物种进行的系统发育分析表明，T. sintenisi 与 Tephritidae 科聚在一起，表明 T. sintenisi 和 Tephritidae 具有密切的系统发育关系。
根据 mitogenome，T. sintenisi 的系统发育关系与传统的形态分类学一致，根据该分类学，T. sintenisi 属于 Otitidae 科，与 Muscidae 科密切相关。
