Aldman A, Lewis D H, Larsson J
Department of Surgery, University Hospital, Linköping, Sweden.
Acta Chir Scand. 1987;153(5-6):345-51.
In nine pigs the arterial blood flow to one hind leg was reduced to 5% of resting values during 8 hours in order to study changes in muscle energy metabolism. Biopsy samples from the gastrocnemius muscle were analyzed for ATP, ADP, AMP, PC, Cr, lactate, pyruvate, glycogen and pH before ischemia, at the end of the ischemic period and during 2.5 hours of reperfusion. During ischemia ATP/TCr decreased to 25%, ECP to 80% and PC/TCr to 6% of preischemic values. Lactate increased eightfold and glycogen fell to 40%. In the reperfusion period, lactate decreased to 1/3 of the end-ischemic value, PC/TCr showed threefold rise and ATP/Cr doubled. TCr fell to 66% of preischemic value. Other changes were not significantly affected by reperfusion. The end-ischemic values indicated severe ischemia, but some improvement of metabolism occurred during early reperfusion. The pig model is suitable for further studies aiming to improve the energy state of the cells during ischemia.
在9头猪身上，为了研究肌肉能量代谢的变化，在8小时内将一条后腿的动脉血流量减少至静息值的5%。在缺血前、缺血期末以及再灌注2.5小时期间，对腓肠肌活检样本进行了ATP、ADP、AMP、磷酸肌酸（PC）、肌酸（Cr）、乳酸、丙酮酸、糖原和pH值的分析。缺血期间，ATP/总肌酸（TCr）降至缺血前值的25%，磷酸肌酸（ECP）降至80%，PC/TCr降至缺血前值的6%。乳酸增加了8倍，糖原降至40%。在再灌注期，乳酸降至缺血期末值的1/3，PC/TCr升高了3倍，ATP/Cr增加了一倍。TCr降至缺血前值的66%。其他变化未受到再灌注的显著影响。缺血期末值表明存在严重缺血，但在早期再灌注期间代谢出现了一些改善。该猪模型适合用于进一步研究旨在改善缺血期间细胞能量状态的方法。
