Wang Xiaoting, Xiao Xinglei
Department of Trauma 2, The Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
Department of Orthopedics, Jiangsu Huai'an Hospital of Traditional Chinese Medicine, Huai'an, China;
Allergol Immunopathol (Madr). 2023 Jan 1;51(1):133-139. doi: 10.15586/aei.v51i1.741. eCollection 2023.
Bone marrow mesenchymal stem cells (BMSCs), with the abilities of multidirectional differentiation and self-renewal, have been widely used in bone repair and regeneration of inflammation-stimulated oral diseases. Galangin is a flavonoid isolated from , exerts anti-obesity, antitumor, and anti-inflammation pharmacological effects. The roles of galangin in lipopolysaccharide-induced inflammation and osteogenic differentiation of BMSCs were investigated.
BMSCs were isolated from rat bone marrow and identified by flow cytometry. The isolated BMSCs were treated with 1 μg/mL lipopolysaccharides or cotreated with lipopolysaccharides and different concentrations of galangin. Cell viability and apoptosis were detected by MTT (tetrazolium component) and flow cytometry. ELISA was used to detect inflammation. Alizarin red staining was used to investigate osteogenic differentiation.
The rat BMSCs showed negative rate of CD34, and positive rate of CD29 and CD44. Lipopolysaccharides treatment reduced cell viability of BMSCs, and promoted the cell apoptosis. Incubation with galangin enhanced cell viability of lipopolysaccharide-stimulated BMSCs, and suppressed the cell apoptosis. Galangin decreased levels of TNF-α, IL-1β, and IL-6 in lipopolysaccharide-stimulated BMSCs through down-regulation of NF-κB phosphorylation (p-NF-κB). Galangin up-regulated expression of osteo-specific proteins, collagen type I alpha 1 (COL1A1), osteopontin (OPN), and runt-related transcription factor 2 (RUNX2), to promote the osteogenic differentiation of lipopolysaccharide-stimulated BMSCs. Protein expression of p-AKT and p-mTOR in lipopolysaccharide-stimulated BMSCs were increased by galangin treatment.
Galangin exerted an anti-inflammatory effect against lipopolysaccharide- stimulated BMSCs and promoted osteogenic differentiation through the activation of AKT/ mTOR signaling.
骨髓间充质干细胞（BMSCs）具有多向分化和自我更新能力，已广泛应用于炎症刺激的口腔疾病的骨修复和再生。高良姜素是一种从[具体来源未给出]中分离出的黄酮类化合物，具有抗肥胖、抗肿瘤和抗炎药理作用。本研究探讨了高良姜素在脂多糖诱导的BMSCs炎症反应和成骨分化中的作用。
从大鼠骨髓中分离BMSCs，并通过流式细胞术进行鉴定。将分离的BMSCs用1μg/mL脂多糖处理或与脂多糖和不同浓度的高良姜素共同处理。采用MTT（四唑盐成分）和流式细胞术检测细胞活力和凋亡情况。用ELISA检测炎症反应。采用茜素红染色研究成骨分化情况。
大鼠BMSCs的CD34阴性率以及CD29和CD44阳性率符合特征。脂多糖处理降低了BMSCs的细胞活力，并促进了细胞凋亡。用高良姜素孵育可增强脂多糖刺激的BMSCs的细胞活力，并抑制细胞凋亡。高良姜素通过下调NF-κB磷酸化（p-NF-κB）降低了脂多糖刺激的BMSCs中TNF-α、IL-1β和IL-6的水平。高良姜素上调了骨特异性蛋白I型胶原α1（COL1A1）、骨桥蛋白（OPN）和 runt相关转录因子2（RUNX2）的表达，以促进脂多糖刺激的BMSCs的成骨分化。高良姜素处理增加了脂多糖刺激的BMSCs中p-AKT和p-mTOR的蛋白表达。
高良姜素对脂多糖刺激的BMSCs具有抗炎作用，并通过激活AKT/mTOR信号通路促进成骨分化。
