利用衰减全反射傅里叶变换红外光谱法研究黄曲霉毒素M1与乳蛋白的相互作用
Interaction study of aflatoxin M1 with milk proteins using ATR-FTIR.
作者信息
Harshitha C G, Sharma Neelima, Singh Richa, Sharma Rajan, Gandhi Kamal, Mann Bimlesh
机构信息
Dairy Chemistry Division, NDRI, Karnal, India.
出版信息
J Food Sci Technol. 2023 Jan;60(1):64-72. doi: 10.1007/s13197-022-05587-x. Epub 2022 Sep 15.
UNLABELLED
Aflatoxin M (AFM) is a metabolite of carcinogenic aflatoxin B (AFB) and appears in milk of dairy animals on ingestion of feed contaminated with AFB. It has been reported to have affinity towards milk proteins, the exact mechanism of which is still unknown. In the present study, ATR-FTIR coupled with chemometrics is utilized to understand AFM interaction with milk proteins. The second order derivative spectra of the spectral window 1700-1600 cm confirms the affinity of AFM towards milk proteins. The results of principal component analysis suggested that spectral window of 1700-1600 cm is informative and provides an indication of the conformational changes brought by AFM in the secondary structure of milk proteins.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13197-022-05587-x.
未标注
黄曲霉毒素M（AFM）是致癌性黄曲霉毒素B（AFB）的代谢产物，在食用受AFB污染的饲料的奶牛所产牛奶中出现。据报道，它对乳蛋白具有亲和力，但其确切机制仍不清楚。在本研究中，利用衰减全反射傅里叶变换红外光谱（ATR-FTIR）结合化学计量学来了解AFM与乳蛋白的相互作用。光谱窗口1700 - 1600 cm的二阶导数光谱证实了AFM对乳蛋白的亲和力。主成分分析结果表明，1700 - 1600 cm的光谱窗口具有信息性，并表明了AFM在乳蛋白二级结构中引起的构象变化。
补充信息
在线版本包含可在10.1007/s13197-022-05587-x获取的补充材料。
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