Zhang Lidong, Lang Hedong, Ran Li, Tian Guoliang, Shen Hui, Zhu Jundong, Zhang Qianyong, Yi Long, Mi Mantian
Chongqing Key Laboratory of Nutrition and Food Safety, Research Center for Nutrition and Food Safety, Chongqing Medical Nutrition Research Center, Institute of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, China.
Front Microbiol. 2022 Dec 21;13:1049469. doi: 10.3389/fmicb.2022.1049469. eCollection 2022.
Exercise is reported to play a crucial role in skeletal muscle performance. However, the underlying mechanism is still unknown. Thus, we investigated the effect of high-intensity aerobic exercise on skeletal muscle performance. In this study, the male C57BL/6J mice were accepted by high-intensity aerobic exercise for 8 weeks to establish an exercise model. It was observed that high-intensity aerobic exercise markedly affected the expression of genes in skeletal muscle. Moreover, high-intensity aerobic exercise significantly improved skeletal muscle grip strength and serum testosterone levels. HE staining showed that the cross-sectional area (CSA) of the skeletal muscle was successfully increased after 8 weeks of high-intensity aerobic exercise. Additionally, we found that high-intensity aerobic exercise changed gut microbiota structure by altering the abundance of , , and , which might be related to testosterone production. However, the beneficial effects disappeared after the elimination of the gut microbiota and recovered after fecal microbiota transplantation (FMT) experiments for 1 week. These results indicated that the beneficial effects of high-intensity aerobic exercise on skeletal muscle were partly dependent on the gut microbiota. Our results suggested that long-term high loading intensity of aerobic exercise could improve skeletal muscle performance, which was probably due to the gut microbiota-testosterone axis.
据报道，运动在骨骼肌性能中起着至关重要的作用。然而，其潜在机制仍不清楚。因此，我们研究了高强度有氧运动对骨骼肌性能的影响。在本研究中，雄性C57BL/6J小鼠接受8周的高强度有氧运动以建立运动模型。观察到高强度有氧运动显著影响骨骼肌中基因的表达。此外，高强度有氧运动显著提高了骨骼肌握力和血清睾酮水平。苏木精-伊红染色显示，高强度有氧运动8周后，骨骼肌的横截面积（CSA）成功增加。此外，我们发现高强度有氧运动通过改变、和的丰度来改变肠道微生物群结构，这可能与睾酮的产生有关。然而，在消除肠道微生物群后，有益效果消失，并在粪便微生物群移植（FMT）实验1周后恢复。这些结果表明，高强度有氧运动对骨骼肌的有益作用部分依赖于肠道微生物群。我们的结果表明，长期高负荷强度的有氧运动可以改善骨骼肌性能，这可能是由于肠道微生物群-睾酮轴所致。
