Zhang Lidong, Tian Guoliang, Huang Li, Zhou Min, Zhu Jundong, Yi Long, Mi Mantian
Chongqing Key Laboratory of Nutrition and Food Safety, Research Center for Nutrition and Food Safety, Chongqing Medical Nutrition Research Center, Institute of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, China.
Front Nutr. 2022 Aug 4;9:965180. doi: 10.3389/fnut.2022.965180. eCollection 2022.
Mounting evidence suggested that high loading intensity of exercise might be detrimental to human health, especially the gastrointestinal tract. Pterostilbene (PTE), derived from grapes and blueberries, might reach a high concentration of intestinal contents. Our study aimed to evaluate PTE's ability to prevent the loss of intestinal epithelial barrier in high loading intensity of exercise. The exercise model was established by the forced running of mice. An effective HPLC-UV method was developed to quantify PTE concentration in intestinal content. The mRNA changes were detected by quantitative polymerase chain reaction (qPCR). The structure of intestinal flora was analyzed by 16S rRNA sequencing. The PTE (100 mg/kg/d) could significantly attenuate exercise-induced intestinal epithelial barrier loss. Moreover, the HPLC-UV assay showed that the PTE concentration of intestinal content could last 12 h. Furthermore, the exercise increased the abundance of Alistipes, which was related to lipopolysaccharide (LPS) production but could not be reversed by PTE intervention. Besides, cell experiments showed that PTE could promote the expression of intestinal epithelial tight junction (TJ) molecules . In conclusion, PTE has a significant interest in preventing exercise-induced intestinal damage.
越来越多的证据表明，高强度运动负荷可能对人体健康有害，尤其是对胃肠道。来源于葡萄和蓝莓的紫檀芪（PTE）可能在肠道内容物中达到高浓度。我们的研究旨在评估PTE在高强度运动负荷下预防肠道上皮屏障受损的能力。通过强迫小鼠跑步建立运动模型。开发了一种有效的高效液相色谱-紫外（HPLC-UV）方法来定量肠道内容物中的PTE浓度。通过定量聚合酶链反应（qPCR）检测mRNA变化。通过16S rRNA测序分析肠道菌群结构。PTE（100mg/kg/d）可显著减轻运动诱导的肠道上皮屏障受损。此外，HPLC-UV分析表明肠道内容物中的PTE浓度可持续12小时。此外，运动增加了与脂多糖（LPS）产生相关的阿里斯杆菌属丰度，但PTE干预无法使其逆转。此外，细胞实验表明PTE可促进肠道上皮紧密连接（TJ）分子的表达。总之，PTE在预防运动诱导的肠道损伤方面具有显著意义。
