Bidhan Chandra Krishi Viswavidyalaya, Department of Agricultural Chemicals, Kalyani, Nadia, West Bengal 741235, India.
Bidhan Chandra Krishi Viswavidyalaya, All India Network Project on Pesticide Residues, Department of Agricultural Chemicals, Kalyani, Nadia, West Bengal 741235, India.
J AOAC Int. 2023 Sep 1;106(5):1209-1219. doi: 10.1093/jaoacint/qsad005.
The presence of undesirable substances, including pesticides (xenobiotics) in betel leaf (Piper betel), is a great concern for consumers because it is chewed and consumed directly. To protect the consumer's health, a modified QuEChERS method for monitoring purposes and subsequent decontamination process has been developed.
The goal of this work was to establish a multi-residue analytical method for monitoring nonpermitted organophosphorus pesticide residues in betel leaf, as well as cost-effective cleaning strategies.
The homogenized 15 g samples (20 betel leaf samples collected in West Bengal, India) were extracted with a modified QuEChERS method using acetonitrile, reconstituted to acetone, and finally analyzed by GC-MS/MS. Possible decontamination techniques (such as tap water washing, 2% saltwater washing, and lukewarm water washing) were evaluated.
The limit of detection ranged from 0.003 to 0.005 mg/kg, and limit of quantification was 0.01 mg/kg. Recoveries ranged from 80 to 120% with RSDr 9%. One sample was found to contain three pesticides 4 to 7 times higher than MRLs. Suggested decontamination methods allowed reducing toxic traces below European limits.
The suggested approach is useful for determining pesticide residues in betel leaves quickly. Traditional techniques of processing betel leaves may reduce pesticide residues below regulatory limits.
A multi-residue method and decontamination of pesticides in betel leaf using QuEChERS-GC-MS/MS technology with satisfactory method performance was achieved. Domestic decontamination techniques have a high efficacy in reducing pesticide residues from betel leaves, making them safe for human consumption.
由于人们咀嚼和直接食用槟榔叶，因此其中存在的有害杂质（包括农药），如污染物，引起了消费者的极大关注。为了保护消费者的健康，已经开发出一种改良的 QuEChERS 方法用于监测目的和后续的净化过程。
本工作旨在建立一种用于监测槟榔叶中禁用有机磷农药残留的多残留分析方法，并制定出具有成本效益的净化策略。
将 15 g 均质化样品（从印度西孟加拉邦收集的 20 份槟榔叶样本）用乙腈进行改良的 QuEChERS 提取，用丙酮重新配制，最后用 GC-MS/MS 进行分析。评估了可能的净化技术（如自来水洗涤、2%盐水洗涤和温水洗涤）。
检测限范围为 0.003 至 0.005 mg/kg，定量限为 0.01 mg/kg。回收率在 80%至 120%之间，RSDr 为 9%。一个样本中发现三种农药的含量是 MRLs 的 4 至 7 倍。建议的净化方法可使有毒痕迹减少到低于欧洲限值。
所建议的方法可用于快速测定槟榔叶中的农药残留。传统的槟榔叶加工技术可以将农药残留降低到监管限值以下。
采用 QuEChERS-GC-MS/MS 技术，建立了一种多残留方法和净化技术，可快速检测槟榔叶中的农药残留。国内的净化技术对降低槟榔叶中的农药残留有很高的效果，使其可安全食用。
