Jarvie D R, Heyworth R, Simpson D
University Department of Clinical Chemistry, Royal Infirmary, Edinburgh, UK.
Ann Clin Biochem. 1987 Jul;24 ( Pt 4):364-73. doi: 10.1177/000456328702400405.
Three colorimetric techniques and one enzymatic technique have been compared with an HPLC method for the analysis of salicylate in the plasma of patients after self-poisoning with aspirin. The enzymatic technique was specific but expensive to perform. The colorimetric techniques were simple, cheap and precise. The Keller method, however, was susceptible to interference from endogenous substances. We report a modification of the Keller method that eliminates most of this interference and improves the accuracy of the analysis. The new method is easier and safer to perform under emergency conditions than the Trinder technique.
已将三种比色法和一种酶法与一种高效液相色谱法进行了比较，用于分析阿司匹林自我中毒患者血浆中的水杨酸盐。酶法具有特异性，但操作成本高。比色法简单、便宜且精确。然而，凯勒法易受内源性物质的干扰。我们报告了一种对凯勒法的改进方法，该方法消除了大部分此类干扰并提高了分析的准确性。在紧急情况下，新方法比Trinder法更容易操作且更安全。
