Gaspari F, Locatelli M
Ther Drug Monit. 1987 Jun;9(2):243-7. doi: 10.1097/00007691-198706000-00020.
A simple, rapid, and sensitive method for the determination of aspirin and salicylic acid in plasma of uremic patients is reported. After extraction with hexane, aspirin (ASA) and salicylic acid (SA) were quantified by high-performance liquid chromatography (HPLC) with ultraviolet detection at 229 nm. ASA and SA concentrations and peak-height ratios were linearly related up to 20 micrograms/ml. The lower limit of sensitivity was 0.1 microgram/ml for both compounds. The average recoveries of aspirin and salicylic acid were 27 and 54%, respectively. This procedure offers better performance than do previously described methods. The sample clean-up allows quantitation of a low concentration of aspirin in uremic patients' plasma, commonly rich in interfering endogenous substances.
报道了一种用于测定尿毒症患者血浆中阿司匹林和水杨酸的简单、快速且灵敏的方法。用己烷萃取后，通过在229nm处进行紫外检测的高效液相色谱法（HPLC）对阿司匹林（ASA）和水杨酸（SA）进行定量。ASA和SA的浓度以及峰高比在高达20微克/毫升时呈线性关系。两种化合物的灵敏度下限均为0.1微克/毫升。阿司匹林和水杨酸的平均回收率分别为27%和54%。该方法比先前描述的方法具有更好的性能。样品净化使得能够对尿毒症患者血浆中低浓度的阿司匹林进行定量，尿毒症患者血浆通常富含干扰性内源性物质。
