通过不同分子检测方法检测和定量血浆样本中的 HBV 和 HCV：比较研究。
Detection and quantification of HBV and HCV in plasma samples by means of different molecular assays: Comparative study.
机构信息
Hospital Público da Mariña, Burela, Spain.
Hospital Universitario Clinico San Cecilio, Instituto de Investigacion Ibs.Granada, Granada, Spain.
出版信息
Enferm Infecc Microbiol Clin (Engl Ed). 2024 Jan;42(1):13-16. doi: 10.1016/j.eimce.2022.12.007. Epub 2023 Jan 7.
INTRODUCTION
Viral load is a very useful marker for monitoring patients infected with HBV and HCV. This work compares assays based on transcription-mediated amplification (TMA) and on real-time PCR (RT-PCR) to verify whether they can be interchangeable.
MATERIAL AND METHODS
A bicentric study, in which 147 plasma samples from patients infected with HBV and 229 with HCV were analyzed, was carried out. TMA-based assays (Aptima® HBV Quant and Aptima® HCV Quant Dx, employing Panther system (Hologic®)) and RT-PCR (COBAS® AmpliPrep/COBAS® TaqMan® and COBAS® 6800) were used and the degree of concordance between them was calculated.
RESULTS
Viral load was detected in both systems in 60 (40.82%) HBV samples (median log viral load: COBAS: 2.51IU/mL (IQR 2.20-3.17), Panther: 2.71IU/mL (IQR 2.21-3.22)) and in 39 (16.96%) HCV samples (median log viral load: COBAS: 3.93IU/mL (IQR 2.24-6.01), Panther: 3.80IU/mL (IQR 1.99-6.14)). The agreement between both systems was κ=0.943 for HBV and κ=0.925 for HCV. Comparison of viral load samples detected by both assays showed a hight correlation for HBV (R=0.86) and for HCV (R=0.97).
CONCLUSIONS
Both TMA and RT-PCR based assays may be interchangeable for the management of patients infected with HBV and HCV.
简介
病毒载量是监测乙型肝炎病毒（HBV）和丙型肝炎病毒（HCV）感染者的非常有用的标志物。本研究比较了基于转录介导扩增（TMA）和实时 PCR（RT-PCR）的检测方法，以验证它们是否可以互换。
材料和方法
进行了一项两中心研究，分析了 147 例 HBV 感染患者和 229 例 HCV 感染患者的血浆样本。采用 TMA 检测法（Aptima®HBV Quant 和 Aptima®HCV Quant Dx，采用 Panther 系统（Hologic®））和 RT-PCR（COBAS®AmpliPrep/COBAS®TaqMan®和 COBAS®6800），并计算了它们之间的一致性程度。
结果
两种系统均检测到 60 例（40.82%）HBV 样本中的病毒载量（COBAS 中位数对数病毒载量：2.51IU/mL（IQR 2.20-3.17）， Panther：2.71IU/mL（IQR 2.21-3.22））和 39 例（16.96%）HCV 样本中的病毒载量（COBAS 中位数对数病毒载量：3.93IU/mL（IQR 2.24-6.01）， Panther：3.80IU/mL（IQR 1.99-6.14））。两种系统之间的一致性为 HBV 的 κ=0.943 和 HCV 的 κ=0.925。对两种检测方法检测到的病毒载量样本进行比较，发现 HBV 相关性高（R=0.86），HCV 相关性高（R=0.97）。
结论
基于 TMA 和 RT-PCR 的检测方法可互换用于管理 HBV 和 HCV 感染者。
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