Grosset A, Knapp M L, Mayne P D
Department of Chemical Pathology, Charing Cross and Westminster Medical School, Westminster Hospital, London, UK.
Ann Clin Biochem. 1987 Sep;24 ( Pt 5):513-7. doi: 10.1177/000456328702400516.
The non-dialysable fraction of haemolysate causes an apparent reduction of plasma alkaline phosphatase (ALP) activity using 4-nitrophenylphosphate as substrate. Analyses using four different buffers showed that the decrease in enzyme activity is affected by the buffer used. The percentage reduction in ALP activity is dependent on the initial ALP activity but not on the isoenzyme present. When diethanolamine was used as buffer, sample blanking almost completely compensated for the apparent reduction in enzyme activity. However, when aminomethylpropanol, aminomethylpropanediol and tris-carbonate buffers were used, it appeared that haemolysate reduced the catalytic activity of the enzyme, since sample blank correction had minimal effect on the results.
以4-硝基苯磷酸酯作为底物时，溶血产物的非透析部分会导致血浆碱性磷酸酶（ALP）活性明显降低。使用四种不同缓冲液进行的分析表明，酶活性的降低受所用缓冲液的影响。ALP活性的降低百分比取决于初始ALP活性，而不取决于存在的同工酶。当使用二乙醇胺作为缓冲液时，样品空白几乎完全补偿了酶活性的明显降低。然而，当使用氨基甲基丙醇、氨基甲基丙二醇和三碳酸盐缓冲液时，溶血产物似乎降低了酶的催化活性，因为样品空白校正对结果的影响最小。
