Pekelharing J M, Leijnse B
Clin Chim Acta. 1978 May 2;85(3):335-8. doi: 10.1016/0009-8981(78)90312-1.
Alkaline phosphatase activities were determined in six lots of 2-amino-2-methyl-1-propanol (AMP) and in six lots of diethanolamine (DEA) buffers without preincubation of the sample. There appeared to be differences between the lot numbers in both cases, resulting in a variation in initial activity. When serum samples are preincubated with buffer a loss of activity was observed in 4 out of the 6 AMP buffers. Four human isoenzymes showed varying inactivation during preincubation with AMP buffer. No loss of activity was observed when the preincubation was done with the six DEA buffers. These results indicate that the purity of the commercially-available buffers is quite unsatisfactory.
在未对样品进行预温育的情况下，测定了6批2-氨基-2-甲基-1-丙醇（AMP）和6批二乙醇胺（DEA）缓冲液中的碱性磷酸酶活性。在这两种情况下，不同批次之间似乎存在差异，导致初始活性有所变化。当血清样品与缓冲液预温育时，在6种AMP缓冲液中有4种观察到活性丧失。4种人同工酶在与AMP缓冲液预温育期间表现出不同程度的失活。当用6种DEA缓冲液进行预温育时，未观察到活性丧失。这些结果表明市售缓冲液的纯度相当不令人满意。
