• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

鉴定和验证微 RNA 特征和关键基因在骨肉瘤肺转移中的发展。

Identification and verification of microRNA signature and key genes in the development of osteosarcoma with lung metastasis.

机构信息

Department of Orthopedics, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, P.R. China.

Department of Oocology, the Second Affiliated Hospital of Soochow University, Suzhou, P.R. China.

出版信息

Medicine (Baltimore). 2022 Dec 9;101(49):e32258. doi: 10.1097/MD.0000000000032258.

DOI:10.1097/MD.0000000000032258
PMID:36626488
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9750666/
Abstract

BACKGROUND

Osteosarcoma (OS) is a heterogeneous malignant spindle cell tumor in children under the age of 20. This study aims to research the association between Solute Carrier Family 7 Member 8 (SLC7A8) as well as related genes and OS.

METHOD

OS and normal samples (GSE38698 and GSE85537) were downloaded from Gene Expression Omnibus dataset. The bioinformatics analysis was performed to distinguish 2 differentially expressed genes, prognostic candidate genes and functional enrichment pathway. Immunohistochemistry and quantitative real-time PCR were utilized for further study.

RESULTS

There were 5 DEMs and 10 differentially expressed genes in cancer tissues compared to normal tissues. According to the km-plot software, ARHGEF3, BSN, PQLC3, and SLC7A8 were significantly related to the overall survival of patients with OS. Furthermore, Multivariate analysis included that SLC7A8 was independent risk factors for OS patients. Furthermore, immunohistochemistry and quantitative real-time PCR outcomes indicated that the expression level of SLC7A8 and hsa-miR-506 was differentially expressed in lung metastasis OS tissues and non-metastasis tissues.

CONCLUSION

The prognostic model based on the miRNA-mRNA network could provide predictive significance for prognosis of OS patients, which would be worthy of clinical application. Our results concluded that SLC7A8 may play a key role in the development of OS.

摘要

背景

骨肉瘤（OS）是 20 岁以下儿童中具有异质性的恶性梭形细胞肿瘤。本研究旨在研究溶质载体家族 7 成员 8（SLC7A8）及相关基因与 OS 的关系。

方法

从基因表达综合数据库中下载 OS 和正常样本（GSE38698 和 GSE85537）。进行生物信息学分析以区分 2 个差异表达基因、预后候选基因和功能富集途径。利用免疫组织化学和实时定量 PCR 进行进一步研究。

结果

与正常组织相比，癌症组织中存在 5 个差异表达基因和 10 个差异表达基因。根据 km-plot 软件，ARHGEF3、BSN、PQLC3 和 SLC7A8 与 OS 患者的总生存期显著相关。此外，多变量分析表明 SLC7A8 是 OS 患者的独立危险因素。此外，免疫组织化学和实时定量 PCR 结果表明，SLC7A8 和 hsa-miR-506 的表达水平在肺转移 OS 组织和非转移组织中存在差异。

结论

基于 miRNA-mRNA 网络的预后模型可为 OS 患者的预后提供预测意义，值得临床应用。我们的研究结果表明，SLC7A8 可能在 OS 的发生发展中起关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/f9dc067335d8/medi-101-e32258-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/e6002e00d6ad/medi-101-e32258-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/bddc5506573c/medi-101-e32258-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/5ec5705dbaca/medi-101-e32258-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/5b46b40c1562/medi-101-e32258-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/2b9de0d00a47/medi-101-e32258-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/712850fc8249/medi-101-e32258-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/f9dc067335d8/medi-101-e32258-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/e6002e00d6ad/medi-101-e32258-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/bddc5506573c/medi-101-e32258-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/5ec5705dbaca/medi-101-e32258-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/5b46b40c1562/medi-101-e32258-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/2b9de0d00a47/medi-101-e32258-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/712850fc8249/medi-101-e32258-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ebd/9750666/f9dc067335d8/medi-101-e32258-g007.jpg

相似文献

1
Identification and verification of microRNA signature and key genes in the development of osteosarcoma with lung metastasis.鉴定和验证微 RNA 特征和关键基因在骨肉瘤肺转移中的发展。
Medicine (Baltimore). 2022 Dec 9;101(49):e32258. doi: 10.1097/MD.0000000000032258.
2
ARHGEF3 Associated with Invasion, Metastasis, and Proliferation in Human Osteosarcoma.ARHGEF3与人类骨肉瘤的侵袭、转移和增殖相关。
Biomed Res Int. 2021 Jul 24;2021:3381957. doi: 10.1155/2021/3381957. eCollection 2021.
3
Differential gene expression analysis for osteosarcoma lung metastases.骨肉瘤肺转移的差异基因表达分析。
Cancer Biomark. 2022;33(3):379-387. doi: 10.3233/CBM-210232.
4
Circular RNAs hsa_circ_0032462, hsa_circ_0028173, hsa_circ_0005909 are predicted to promote CADM1 expression by functioning as miRNAs sponge in human osteosarcoma.环状 RNA hsa_circ_0032462、hsa_circ_0028173、hsa_circ_0005909 被预测通过作为 miRNA 海绵在人骨肉瘤中发挥作用来促进 CADM1 的表达。
PLoS One. 2018 Aug 28;13(8):e0202896. doi: 10.1371/journal.pone.0202896. eCollection 2018.
5
Using biological information to analyze potential miRNA-mRNA regulatory networks in the plasma of patients with non-small cell lung cancer.利用生物信息学分析非小细胞肺癌患者血浆中潜在的 miRNA-mRNA 调控网络。
BMC Cancer. 2022 Mar 21;22(1):299. doi: 10.1186/s12885-022-09281-1.
6
Integrated Analysis of Competing Endogenous RNAs Network Reveals Potential Signatures in Osteosarcoma Development.整合分析竞争内源性 RNA 网络揭示骨肉瘤发展中的潜在特征。
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820957025. doi: 10.1177/1533033820957025.
7
miR-491 Inhibits Osteosarcoma Lung Metastasis and Chemoresistance by Targeting αB-crystallin.miR-491 通过靶向 αB-晶状体蛋白抑制骨肉瘤肺转移和化疗耐药性。
Mol Ther. 2017 Sep 6;25(9):2140-2149. doi: 10.1016/j.ymthe.2017.05.018. Epub 2017 Jun 23.
8
Construction of a transcription factor-miRNA-mRNA interactive network elucidates underlying pathogenesis for osteosarcoma and validation by qRT-PCR.构建转录因子-miRNA-mRNA 相互作用网络阐明骨肉瘤的潜在发病机制，并通过 qRT-PCR 进行验证。
Medicine (Baltimore). 2022 Oct 14;101(41):e31049. doi: 10.1097/MD.0000000000031049.
9
Expression of miR‑542‑3p in osteosarcoma with miRNA microarray data, and its potential signaling pathways.miR-542-3p 在 miRNA 微阵列数据中的表达与骨肉瘤及其潜在信号通路
Mol Med Rep. 2019 Feb;19(2):974-983. doi: 10.3892/mmr.2018.9761. Epub 2018 Dec 13.
10
miRNA-142-3p functions as a potential tumor suppressor directly targeting FAM83D in the development of ovarian cancer.miRNA-142-3p 通过直接靶向 FAM83D 在卵巢癌的发展中发挥潜在的肿瘤抑制作用。
Aging (Albany NY). 2022 Apr 22;14(8):3387-3399. doi: 10.18632/aging.203998.

引用本文的文献

1
Non-Coding RNAs in Diagnostic Pathology of High-Grade Central Osteosarcoma.非编码RNA在高级别中央型骨肉瘤诊断病理学中的应用
Diagnostics (Basel). 2025 May 28;15(11):1355. doi: 10.3390/diagnostics15111355.

本文引用的文献

1
ARHGEF3 Associated with Invasion, Metastasis, and Proliferation in Human Osteosarcoma.ARHGEF3与人类骨肉瘤的侵袭、转移和增殖相关。
Biomed Res Int. 2021 Jul 24;2021:3381957. doi: 10.1155/2021/3381957. eCollection 2021.
2
Identification of the miRNA signature and key genes in colorectal cancer lymph node metastasis.结直肠癌淋巴结转移中miRNA特征及关键基因的鉴定
Cancer Cell Int. 2021 Jul 7;21(1):358. doi: 10.1186/s12935-021-02058-9.
3
HS3ST2 and Its Related Molecules as Potential Biomarkers for Predicting Lymph Node Metastasis in Patients with Colorectal Cancer.
HS3ST2及其相关分子作为预测结直肠癌患者淋巴结转移的潜在生物标志物
Onco Targets Ther. 2021 Jun 25;14:3881-3894. doi: 10.2147/OTT.S311038. eCollection 2021.
4
High Expression of N-Acetylgalactosaminyl-transferase 1 (GALNT1) Associated with Invasion, Metastasis, and Proliferation in Osteosarcoma.N-乙酰半乳糖胺基转移酶1（GALNT1）的高表达与骨肉瘤的侵袭、转移和增殖相关。
Med Sci Monit. 2020 Dec 7;26:e927837. doi: 10.12659/MSM.927837.
5
LncRNA MALAT1 facilitates lung metastasis of osteosarcomas through miR-202 sponging.长链非编码 RNA MALAT1 通过海绵吸附 miR-202 促进骨肉瘤的肺转移。
Sci Rep. 2020 Jul 29;10(1):12757. doi: 10.1038/s41598-020-69574-y.
6
MicroRNA-134 inhibits tumor stem cell migration and invasion in oral squamous cell carcinomas via downregulation of PI3K-Akt signaling pathway by inhibiting LAMC2 expression.MicroRNA-134 通过抑制 LAMC2 的表达，下调 PI3K-Akt 信号通路，抑制口腔鳞状细胞癌肿瘤干细胞的迁移和侵袭。
Cancer Biomark. 2020;29(1):51-67. doi: 10.3233/CBM-191362.
7
MicroRNA-24 inhibits the proliferation, migration and invasion and enhances chemosensitivity of human gastric cancer by targeting DND1.微小 RNA-24 通过靶向 DND1 抑制人胃癌的增殖、迁移和侵袭并增强化疗敏感性。
J BUON. 2020 Mar-Apr;25(2):1001-1006.
8
APEC infection affects cytokine-cytokine receptor interaction and cell cycle pathways in chicken trachea.APEC 感染影响鸡气管中的细胞因子-细胞因子受体相互作用和细胞周期途径。
Res Vet Sci. 2020 Jun;130:144-152. doi: 10.1016/j.rvsc.2020.03.016. Epub 2020 Mar 10.
9
Identification of Hub Genes and Pathways in Gastric Adenocarcinoma Based on Bioinformatics Analysis.基于生物信息学分析的胃腺癌关键基因和通路的鉴定。
Med Sci Monit. 2020 Feb 14;26:e920261. doi: 10.12659/MSM.920261.
10
Cancer as a disease of old age: changing mutational and microenvironmental landscapes.癌症作为一种老年疾病：不断变化的突变和微环境景观。
Br J Cancer. 2020 Mar;122(7):943-952. doi: 10.1038/s41416-019-0721-1. Epub 2020 Feb 11.