Chen Hauh-Jyun Candy
Department of Chemistry and Biochemistry and Center for Nano Bio-Detection (AIM-HI), National Chung Cheng University, 168 University Road, Ming-Hsiung, Chia-Yi 62142, Taiwan.
Chem Res Toxicol. 2023 Feb 20;36(2):132-140. doi: 10.1021/acs.chemrestox.2c00354. Epub 2023 Jan 10.
Acrolein is a major component in cigarette smoke and a product of endogenous lipid peroxidation. It is difficult to distinguish human exposure to acrolein from exogenous sources versus endogenous causes, as components in cigarette smoke can stimulate lipid peroxidation in vivo. Therefore, analysis of acrolein-induced DNA and protein adducts by the highly accurate, sensitive, and specific mass spectrometry-based methods is vital to estimate the degree of damage by this IARC Group 2A carcinogen. This Perspective reviews the analyses of acrolein-induced DNA and protein adducts in humans by mass spectrometry focusing on samples accessible for biomonitoring, including DNA from leukocytes and oral cells and abundant proteins from blood, i.e., hemoglobin and serum albumin.
丙烯醛是香烟烟雾中的主要成分，也是内源性脂质过氧化的产物。由于香烟烟雾中的成分可刺激体内脂质过氧化，因此很难区分人体接触丙烯醛是源于外源性还是内源性。因此，采用基于质谱的高度准确、灵敏且特异的方法分析丙烯醛诱导的DNA和蛋白质加合物，对于评估这种国际癌症研究机构2A类致癌物造成的损害程度至关重要。本综述聚焦于可用于生物监测的样本，即白细胞和口腔细胞中的DNA以及血液中的丰富蛋白质（即血红蛋白和血清白蛋白），对通过质谱法分析人体中丙烯醛诱导的DNA和蛋白质加合物进行了综述。
