Jamialahmadi Khadijeh, Amiri Amir Hossein, Zahedipour Fatemeh, Faraji Fahimeh, Karimi Gholamreza
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
J Pharmacopuncture. 2022 Dec 31;25(4):344-353. doi: 10.3831/KPI.2022.25.4.344.
Auraptene is the most abundant natural prenyloxycoumarin. Recent studies have shown that it has multiple biological and therapeutic properties, including antioxidant properties. Erythrocytes are constantly subjected to oxidative damage that can affect proteins and lipids within the erythrocyte membrane and lead to some hemoglobinopathies. Due to the lack of sufficient information about the antioxidant effects of auraptene on erythrocytes, this study intended to evaluate the potential of this compound in protecting radical-induced erythrocytes damages.
The antioxidant activity of auraptene was measured based on DPPH and FRAP assays. Notably, oxidative hemolysis of human erythrocytes was used as a model to study the ability of auraptene to protect biological membranes from free radical-induced damage. Also, the effects of auraptene in different concentrations (25-400 µM) on AAPH-induced lipid/protein peroxidation, glutathione (GSH) content and morphological changes of erythrocytes were determined.
Oxidative hemolysis and lipid/protein peroxidation of erythrocytes were significantly suppressed by auraptene in a time and concentration-dependent manner. Auraptene prevented the depletion of the cytosolic antioxidant GSH in erythrocytes. Furthermore, it inhibited lipid and protein peroxidation in a time and concentration-dependent manner. Likewise, FESEM results demonstrated that auraptene reduced AAPH-induced morphological changes in erythrocytes.
Auraptene efficiently protects human erythrocytes against free radicals. Therefore, it can be a potent candidate for treating oxidative stress-related diseases.
奥勒皮素是最丰富的天然异戊烯氧基香豆素。最近的研究表明它具有多种生物学和治疗特性，包括抗氧化特性。红细胞不断受到氧化损伤，这种损伤会影响红细胞膜内的蛋白质和脂质，并导致一些血红蛋白病。由于缺乏关于奥勒皮素对红细胞抗氧化作用的足够信息，本研究旨在评估该化合物保护自由基诱导的红细胞损伤的潜力。
基于DPPH和FRAP测定法测量奥勒皮素的抗氧化活性。值得注意的是，使用人红细胞的氧化溶血作为模型来研究奥勒皮素保护生物膜免受自由基诱导损伤的能力。此外，还测定了不同浓度（25 - 400 μM）的奥勒皮素对AAPH诱导的红细胞脂质/蛋白质过氧化、谷胱甘肽（GSH）含量和形态变化的影响。
奥勒皮素以时间和浓度依赖性方式显著抑制红细胞的氧化溶血和脂质/蛋白质过氧化。奥勒皮素可防止红细胞胞质抗氧化剂GSH的消耗。此外，它以时间和浓度依赖性方式抑制脂质和蛋白质过氧化。同样，场发射扫描电子显微镜（FESEM）结果表明，奥勒皮素减少了AAPH诱导的红细胞形态变化。
奥勒皮素能有效保护人红细胞免受自由基侵害。因此，它可能是治疗氧化应激相关疾病的有力候选药物。
