M.M. Chengdu University of Traditional Chinese Medicine - Department of Nephrology - Affiliated Integrated TCM and Western Medicine Hospital of Chengdu - Chengdu Integrated TCM and Western Medicine Hospital - Chengdu First People's Hospital - Chengdu, China.
M.M. Chengdu University of Traditional Chinese Medicine - Department of Nephrology - Hospital of Chengdu - Chengdu, China.
Acta Cir Bras. 2023 Jan 6;37(10):e371003. doi: 10.1590/acb371003. eCollection 2023.
To explore the potential impact of traditional Chinese herb FuZhengHuaYuJiangZhuTongLuo recipe (FZHY) on renal interstitial fibrosis (RIF) in chronic kidney disease (CKD) at cellular and molecular levels.
Unilateral ureteral obstruction (UUO) rats were established as the RIF model in vivo. The rats were given intragastric administration with FZHY once a day for consecutive 7, 14 and 21 days, respectively. The renal function parameters and inflammation indicators in kidney tissues were measured using enzyme-linked immunosorbent assay, the CD4+/CD8+ T cells in peripheral blood was detected using flow cytometry, the renal fibrosis degree was estimated using Masson's staining, and the fibrosis-related genes' expression was detected using quantitative polymerase chain reaction, western blotting, and immunohistochemistry analyses.
FZHY prescription reduced the serum creatinine and blood urea nitrogen, decreased the levels of c-reactive protein, interleukin-1, interleukin-6 and tumor necrosis factor-α in kidney tissues, and increased the ratio of CD4+/CD8+ T cells in peripheral blood. FZHY prescription suppressed the renal tissue fibrosis and reduced the levels of laminin, fibronectin, collagen I and collagen III.
FZHY prescription suppressed the renal fibrosis and improved the condition of "Healthy Qi Deficiency and Evil Qi Excess" in rats with UUO, which may provide an effective method for CKD treatment.
从细胞和分子水平探讨扶正化瘀降浊通络方对慢性肾脏病（CKD）肾间质纤维化（RIF）的潜在影响。
采用单侧输尿管梗阻（UUO）大鼠建立 RIF 模型，体内实验中，将大鼠分别给予扶正化瘀降浊通络方灌胃 7、14 和 21 天，酶联免疫吸附试验检测肾脏组织中肾功能参数和炎症指标，流式细胞术检测外周血中 CD4+/CD8+T 细胞，马松染色评估肾纤维化程度，实时定量聚合酶链反应、蛋白质印迹和免疫组织化学分析检测纤维化相关基因的表达。
扶正化瘀降浊通络方降低了血清肌酐和血尿素氮，降低了肾脏组织中 C 反应蛋白、白细胞介素-1、白细胞介素-6 和肿瘤坏死因子-α的水平，增加了外周血中 CD4+/CD8+T 细胞的比例。扶正化瘀降浊通络方抑制了肾组织纤维化，降低了层粘连蛋白、纤维连接蛋白、I 型和 III 型胶原的水平。
扶正化瘀降浊通络方抑制了 UUO 大鼠的肾纤维化，改善了“正气不足，邪气有余”的状况，为 CKD 的治疗提供了一种有效的方法。
