Ahmed Sajad, Chouhan Rekha, Junaid Alim, Jamwal Vijay Lakshmi, Thakur Jitendra, Mir Bilal Ahmad, Gandhi Sumit G
Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, Jammu and Kashmir, India.
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, 143005, Punjab, India.
Funct Integr Genomics. 2023 Jan 11;23(1):35. doi: 10.1007/s10142-023-00961-0.
Rohitukine is a chromone alkaloid and precursor of potent anticancer drugs flavopiridol, P-276-00, and 2,6-dichloro-styryl derivative (11d) (IIIM-290). The metabolite is reported to possess anticancer, anti-inflammatory, antiadipogenic, immunomodulatory, gastroprotective, anti-implantation, antidyslipidemic, anti-arthritic, and anti-fertility properties. However, the physiological role of rohitukine in plant system is yet to be explored. Here, we studied the effect of rohitukine isolated from Dysoxylum gotadhora on Arabidopsis thaliana. The A. thaliana plants grown on a medium fortified with different rohitukine concentrations showed a significant effect on the growth and development. The root growth of A. thaliana seedlings showed considerable inhibition when grown on medium containing 1.0 mM of rohitukine. Transcriptomic analysis indicated the expression of 895 and 932 genes in control and treated samples respectively at a cut-off of FPKM ≥ 1 and P-value < 0.05. Gene ontology (GO) analysis revealed the upregulation of genes related to photosynthesis, membrane transport, antioxidation, xenobiotic degradation, and some transcription factors (TFs) in response to rohitukine. Conversely, rohitukine downregulated several genes including RNA helicases and those involved in nitrogen compound metabolism. The RNA-seq result was also validated by real-time qRT-PCR analysis. In light of these results, we discuss (i) likely ecological importance of rohitukine in parent plant as well as (ii) comparison between responses to rohitukine treatment in plants and mammals.
洛希图碱是一种色酮生物碱，也是强效抗癌药物黄酮哌啶醇、P - 276 - 00和2,6 - 二氯苯乙烯衍生物（11d）（IIIM - 290）的前体。据报道，该代谢产物具有抗癌、抗炎、抗脂肪生成、免疫调节、胃保护、抗着床、抗血脂异常、抗关节炎和抗生育等特性。然而，洛希图碱在植物系统中的生理作用尚待探索。在此，我们研究了从毛瓣楝中分离出的洛希图碱对拟南芥的影响。在添加了不同浓度洛希图碱的培养基上生长的拟南芥植株，其生长和发育受到了显著影响。当在含有1.0 mM洛希图碱的培养基上生长时，拟南芥幼苗的根生长受到了相当大的抑制。转录组分析表明，在FPKM≥1且P值<0.05的阈值下，对照样品和处理样品中分别有895个和932个基因表达。基因本体（GO）分析显示，响应洛希图碱，与光合作用、膜转运、抗氧化、异源生物降解和一些转录因子（TFs）相关的基因上调。相反，洛希图碱下调了包括RNA解旋酶和参与氮化合物代谢的基因在内的几个基因。RNA测序结果也通过实时定量逆转录 - 聚合酶链反应（qRT - PCR）分析得到了验证。鉴于这些结果，我们讨论了（i）洛希图碱在亲本植物中可能的生态重要性，以及（ii）植物和哺乳动物对洛希图碱处理反应的比较。
