Mikkelsen J, Højrup P, Nielsen P F, Roepstorff P, Knudsen J
Institute of Biochemistry, University of Odense, Denmark.
Biochem J. 1987 Aug 1;245(3):857-61. doi: 10.1042/bj2450857.
Acyl-CoA-binding protein from bovine liver was purified with the use of reverse-phase h.p.l.c. in the final step. The complete amino acid sequence was determined by using a combination of gas-phase Edman degradation and electron-impact and fast-atom-bombardment mass spectrometry. The sequence was confirmed by determination of the Mr by plasma-desorption time-of-flight mass spectrometry.
牛肝酰基辅酶A结合蛋白在最后一步通过反相高效液相色谱法进行纯化。完整的氨基酸序列通过气相埃德曼降解法与电子轰击和快原子轰击质谱法相结合来确定。该序列通过等离子体解吸飞行时间质谱法测定相对分子质量得到证实。