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N-聚糖硫酸化纤连蛋白:培养的内皮细胞合成的几种硫酸化糖蛋白之一。

N-glycansulfated fibronectin: one of the several sulfated glycoproteins synthesized by endothelial cells in culture.

作者信息

Colburn P, Buonassisi V, Dietrich C P, Nader H B

机构信息

W.A.J. Cell Science Center, Lake Placid, N.Y. 12946.

出版信息

Biochem Biophys Res Commun. 1987 Sep 30;147(3):920-6. doi: 10.1016/s0006-291x(87)80158-4.

Abstract

N-glycanase, an endoglycosidase that cleaves the bond between asparagine and glucosamine, releases oligosaccharides with various degree of sulfation from endothelial cell fibronectin. As shown by analysis by polyacrylamide gel electrophoresis of culture medium conditioned by cells exposed to [35S]sulfate, endothelial cell fibronectin is one of a number of glycoproteins bearing sulfated oligosaccharides, synthesized by this cell type.

摘要

N-聚糖酶是一种内切糖苷酶,可切割天冬酰胺与葡糖胺之间的键,从内皮细胞纤连蛋白释放出不同硫酸化程度的寡糖。如对暴露于[35S]硫酸盐的细胞所分泌的培养基进行聚丙烯酰胺凝胶电泳分析所示,内皮细胞纤连蛋白是由这种细胞类型合成的多种带有硫酸化寡糖的糖蛋白之一。

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