Bhat N R, Brunngraber E G
Biochem Biophys Res Commun. 1985 Jan 31;126(2):778-84. doi: 10.1016/0006-291x(85)90252-9.
Both primary cultured glial cells and cloned (C-6) glioma cells have been shown to synthesize and release sulfated glycoproteins. It was found that N-linked tri- and tetra-antennary glycopeptides recovered from the glycoproteins contained most of the (35S) sulfate label. C-6 glial cells showed a higher rate of oligosaccharide sulfation than the primary glial cultures. Both cell types exhibited a high rate of release of sulfated glycoproteins into the medium. The ratio of 35S/3H incorporated from (35S) sulfate and (3H) glucosamine in the released material was higher than that of the glycoproteins associated with the cell, indicating an enrichment of sulfated glycoproteins in the secreted materials. Monensin inhibited both the synthesis and the release of sulfated glycoproteins.
原代培养的神经胶质细胞和克隆的（C-6）胶质瘤细胞均已被证明能合成并释放硫酸化糖蛋白。研究发现，从糖蛋白中回收的N-连接三触角和四触角糖肽含有大部分（35S）硫酸盐标记。C-6神经胶质细胞的寡糖硫酸化速率高于原代神经胶质细胞培养物。两种细胞类型都表现出将硫酸化糖蛋白大量释放到培养基中的现象。释放物质中由（35S）硫酸盐和（3H）葡糖胺掺入的35S/3H比率高于与细胞相关的糖蛋白，这表明分泌物质中硫酸化糖蛋白有所富集。莫能菌素抑制硫酸化糖蛋白的合成和释放。
