Graduate Program in Environmental Engineering, Federal University of Ouro Preto, Campus Morro do Cruzeiro, Ouro Preto, Brazil.
Graduate Program in Biotechnology, Federal University of Ouro Preto, Campus Morro do Cruzeiro, Ouro Preto, Brazil.
Environ Technol. 2024 Apr;45(11):2205-2217. doi: 10.1080/09593330.2023.2166877. Epub 2023 Jan 20.
In anaerobic digestion (AD), the choice of inoculum type seems to be relevant for methane production for complex substrates, such as lignocellulosic material. Previous work demonstrated that the addition of fresh manure and ruminal fluid to anaerobic sludge improved methane productivity and kinetics of AD of crude sugarcane bagasse (CSB). Considering that the improvement of methane production could be a result of a more adapted microbial community, the present study performed the Next Generation Sequencing analysis to identify changes in the microbiome of anaerobic sludge inoculum, resulting from fresh manure and ruminal fluid addition. In comparison with AD performed only with anaerobic sludge inoculum (50:50, U), accumulated methane production was 15% higher with anaerobic sludge plus ruminal fluid inoculum (50:50, UR) and even higher (68%) with anaerobic sludge with fresh bovine manure inoculum (50:50, UFM), reaching the value of 143 NmLg. species were highly abundant in all inocula, playing an important role during the hydrolysis and fermentation of CSB, and detoxifying potential inhibitors. Microbial composition also revealed the occurrence of and at UFM inoculum that seem to have contributed to the higher methane production rate, mainly due to their hydrolytic and fermentative ability on lignocellulosic substrates. On the other hand, the presence of might have had a negative effect on methane production due to their ability to perform methane oxidation.
在厌氧消化（AD）中，接种物类型的选择对于复杂底物（如木质纤维素材料）的甲烷生产似乎是相关的。先前的工作表明，向厌氧污泥中添加新鲜粪便和瘤胃液可提高粗甘蔗渣（CSB）AD 的甲烷产率和动力学。考虑到甲烷产量的提高可能是由于微生物群落更加适应，本研究通过下一代测序分析来确定新鲜粪便和瘤胃液添加对厌氧污泥接种物微生物组的变化。与仅用厌氧污泥接种物（50:50，U）进行的 AD 相比，厌氧污泥加瘤胃液接种物（50:50，UR）的累积甲烷产量提高了 15%，而厌氧污泥加新鲜牛粪接种物（50:50，UFM）的累积甲烷产量甚至更高（68%），达到 143 NmLg。在所有接种物中， 菌门高度丰富，在 CSB 的水解和发酵过程中发挥重要作用，并具有潜在抑制剂的解毒能力。微生物组成还揭示了 UFM 接种物中 菌门和 菌门的存在，它们似乎有助于提高甲烷产率，主要是由于它们对木质纤维素底物的水解和发酵能力。另一方面，由于其进行甲烷氧化的能力， 菌门的存在可能对甲烷生产产生负面影响。
