Sakurai Riku, Yokoyama Yoshimi, Fukuda Yasuhiro, Kawakami Masaki, Hashimoto Satoshi, Tada Chika
Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, Japan.
Japan Society for the Promotion of Science, Tokyo 980-8577, Japan.
ACS Omega. 2024 Sep 12;9(38):39643-39651. doi: 10.1021/acsomega.4c03998. eCollection 2024 Sep 24.
Long-chain fatty acid (LCFA) degradation primarily involves several species of and hydrogenotrophic methanogens, constituting the rate-limiting step in anaerobic digestion. It is crucial to augment their abundance to enhance LCFA degradation. Utilizing microbial carriers presents an effective strategy to maintain the microorganisms on the surface and prevent their washout from the digester. In this study, we aimed to identify a suitable microbial carrier with a superior adsorption capacity for LCFA-degrading microorganisms. We tested various polymers, poly(vinyl alcohol) (PVA), polypropylene (PP), polyethylene glycol (PEG), and polyvinylidene chloride (PVDC), adding them to the sludge at the concentration of 28.25 g L and incubating with olive oil. The amplicon sequencing analysis revealed that PVDC retained more abundantly than the other polymers. Remarkably, PVDC predominantly adsorbed LCFA-degrading and , whereas medium- to short-chain fatty acid-degrading was abundant in the sludge. Moreover, hydrogenotrophic was detected at 2.3-9.5 times higher abundance on PVDC compared to the sludge. Further analysis indicated that not only these LCFA-degrading syntrophic microbial communities but also and , which are capable of lipid hydrolysis and glycerol degradation, became dominant on PVDC. Actually, chemical analysis confirmed that adding PVDC promoted the olive oil degradation. These results underscore the potential of PVDC in promoting anaerobic LCFA degradation.
长链脂肪酸（LCFA）降解主要涉及几种 和氢营养型产甲烷菌，这是厌氧消化中的限速步骤。增加它们的丰度对于增强LCFA降解至关重要。利用微生物载体是一种有效的策略，可以将微生物维持在表面并防止它们从消化池中被冲走。在本研究中，我们旨在确定一种对LCFA降解微生物具有优异吸附能力的合适微生物载体。我们测试了各种聚合物，聚乙烯醇（PVA）、聚丙烯（PP）、聚乙二醇（PEG）和聚偏二氯乙烯（PVDC），以28.25 g/L的浓度将它们添加到污泥中并与橄榄油一起培养。扩增子测序分析表明，PVDC比其他聚合物保留得更丰富。值得注意的是，PVDC主要吸附LCFA降解 和 ，而中短链脂肪酸降解 在污泥中含量丰富。此外，与污泥相比，在PVDC上检测到氢营养型 的丰度高2.3至9.5倍。进一步分析表明，不仅这些LCFA降解共生微生物群落，而且能够进行脂质水解和甘油降解的 和 在PVDC上也占主导地位。实际上，化学分析证实添加PVDC促进了橄榄油的降解。这些结果强调了PVDC在促进厌氧LCFA降解方面的潜力。
