Wang Xinrui, Lu Jianzhong, Li Guotong, Luo Lifei, Yuan Zhen, Li Min, Zhang Jingze, Liu Dailin
State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China; Tianjin Modern Innovation Chinese Medicine Technology Co., Ltd., Tianjin, China.
Tianjin Modern Innovation Chinese Medicine Technology Co., Ltd., Tianjin, China.
J Ethnopharmacol. 2023 Apr 6;305:116094. doi: 10.1016/j.jep.2022.116094. Epub 2023 Jan 9.
As one of China's 100 classic recipes, Taohong Siwu Decoction (THSWD) consists of Siwu Tang flavored peach kernel and safflower, and is used to nourish and activate blood. Accordingly, THSWD is mainly administered to treat blood deficiency and stasis syndrome. According to prior studies, THSWD induces antioxidant stress, inhibits inflammatory reactions, inhibits platelet aggregation, prevents fibrosis, reduces blood lipids, prolongs clotting time, prevents atherosclerosis and vascular pathology, improves hemorheological changes, and regulates related signaling pathways.
A sensitive analytical method was developed to detect the marker components of THSWD using UPLC-Q-TOF-MS. A rapid and sensitive UPLC-MS/MS analytical method was developed and applied to detect 16 major bioactive components in normal and acute blood stasis (ABS) rats following oral administration of THSWD. The metabolic process of THSWD in vivo was evaluated and the differences in pharmacokinetic parameters between the normal and ABS rat metabolic processes were compared.
This method was fully validated based on its excellent linearity (r < 0.99), satisfactory intra- and inter-day precisions (RSD <15%), and good accuracy (RE within ±14.83%). The stability, matrix effects, and extraction recoveries of the rat plasma samples were also within the acceptable limits (RSD <15%). Compared to normal rats, the pharmacokinetics of the major active constituents (except Senkyunolide G) were significantly different (P < 0.05) in the ABS model rats, indicating that the metabolism of the 16 compounds in vivo may change under disease conditions.
In this study, a sensitive UPLC-Q-TOF-MS method was established to analyze the main components of THSWD, and a UPLC-MS/MS analytical method was developed and applied for the pharmacokinetic parameter detection of the 16 main bioactive components in normal and ABS rats. Our findings lay the foundation for further studies on the pharmacokinetic-pharmacodynamic correlation for THSWD.
作为中国100个经典方剂之一，桃红四物汤由四物汤加味桃仁和红花组成，用于养血活血。因此，桃红四物汤主要用于治疗血虚血瘀证。根据先前的研究，桃红四物汤可诱导抗氧化应激、抑制炎症反应、抑制血小板聚集、预防纤维化、降低血脂、延长凝血时间、预防动脉粥样硬化和血管病变、改善血液流变学变化并调节相关信号通路。
建立了一种灵敏的分析方法，采用超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF-MS）检测桃红四物汤的标志性成分。开发并应用了一种快速灵敏的超高效液相色谱-串联质谱（UPLC-MS/MS）分析方法，用于检测正常和急性血瘀（ABS）大鼠口服桃红四物汤后16种主要生物活性成分。评估了桃红四物汤在体内的代谢过程，并比较了正常和ABS大鼠代谢过程中药代动力学参数的差异。
该方法具有良好的线性（r < 0.99）、令人满意的日内和日间精密度（RSD < 15%）以及良好的准确度（RE在±14.83%以内），从而得到了充分验证。大鼠血浆样品的稳定性、基质效应和提取回收率也在可接受范围内（RSD < 15%）。与正常大鼠相比，主要活性成分（除藁本内酯G外）在ABS模型大鼠中的药代动力学有显著差异（P < 0.05），表明这16种化合物在体内的代谢可能在疾病状态下发生变化。
本研究建立了一种灵敏的UPLC-Q-TOF-MS方法来分析桃红四物汤的主要成分，并开发了一种UPLC-MS/MS分析方法用于检测正常和ABS大鼠中16种主要生物活性成分的药代动力学参数。我们的研究结果为进一步研究桃红四物汤的药代动力学-药效学相关性奠定了基础。
