Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, No. 12 Jichang Road, Guangzhou, 510405, Guangdong Province, China.
School of TCM Healthcare, Guangdong Food and Drug Vocational College, Guangzhou, 510520, China.
Eur J Drug Metab Pharmacokinet. 2023 May;48(3):301-310. doi: 10.1007/s13318-023-00825-9. Epub 2023 Apr 20.
Taohong Siwu Decoction (TSD) is a classic traditional Chinese medicine (TCM) compound with pharmacological effects such as vasodilation and hypolipidemia. Paeoniflorin (PF) is one of the active ingredients of TSD. The aim of this study was to evaluate the pharmacokinetics of PF in herbal extracts and their purified forms in rats.
A sensitive and rapid high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method for the determination of PF in rat plasma was developed. Rats were divided into three groups, and given PF solution, water extract of white peony root (WPR), or TSD by gavage. At different predetermined timepoints after gavage, blood was collected from the orbital vein. The pharmacokinetic parameters of PF in the plasma of rats in the three groups was determined.
The pharmacokinetic studies showed that the time to reach maximum concentration (T) of PF in the purified forms group was relatively high, while the half-lives (T) of PF in the TSD and WPR groups were longer. Among the three groups, PF in the purified forms group had the maximum area under the concentration-time curve (AUC = 732.997 µg/L·h) and the largest maximum concentration (C = 313.460 µg/L), which showed a significant difference compared with the TSD group (P < 0.05). Compared with the purified group, the clearance (CL/F = 86.004 L/h/kg) and the apparent volume of distribution (V/F = 254.787 L/kg) of PF in the TSD group increased significantly (P < 0.05).
A highly specific, sensitive, and rapid HPLC-MS-MS method was developed and applied for the determination of PF in rat plasma. It was found that TSD and WPR can prolong the action time of paeoniflorin in the body.
桃红四物汤（TSD）是一种具有扩张血管和降血脂等药理作用的经典中药（TCM）复方。芍药苷（PF）是 TSD 的一种活性成分。本研究旨在评估 PF 在草药提取物及其纯化形式中的药代动力学在大鼠体内的情况。
建立了一种灵敏、快速的高效液相色谱-串联质谱（HPLC-MS-MS）测定大鼠血浆中 PF 的方法。将大鼠分为三组，分别灌胃给予 PF 溶液、白芍水提物（WPR）和 TSD。在灌胃后不同的预定时间点，从眶静脉采集血液。测定三组大鼠血浆中 PF 的药代动力学参数。
药代动力学研究表明，纯化形式组 PF 的达峰时间（T）相对较高，而 TSD 和 WPR 组的 PF 半衰期（T）较长。在这三组中，PF 在纯化形式组的曲线下面积（AUC=732.997μg/L·h）最大，最大浓度（C=313.460μg/L）最大，与 TSD 组相比有显著差异（P<0.05）。与纯化组相比，TSD 组 PF 的清除率（CL/F=86.004 L/h/kg）和表观分布容积（V/F=254.787 L/kg）明显增加（P<0.05）。
建立并应用了一种高效、灵敏、快速的 HPLC-MS-MS 法测定大鼠血浆中的 PF。结果表明，TSD 和 WPR 可以延长 PF 在体内的作用时间。
