Zhang Bingyan, Wang Yunping, Lu Rongrong
School of Food Science and Technology, Jiangnan University, Wuxi, China.
J Sci Food Agric. 2023 May;103(7):3579-3591. doi: 10.1002/jsfa.12447. Epub 2023 Jan 24.
In recent years, the design of food-grade Pickering emulsion delivery systems has become an effective strategy for improving the low bioavailability of bioactive substances. Protein-based Pickering emulsions have received extensive attention because of a high biocompatibility and loading capacity. The bioavailability of active substances is mainly evaluated by simulating in vitro gastrointestinal digestion. As a model organism for antioxidation and anti-aging, Caenorhabditis elegans can provide additional biological information for the in vivo utilization of active substances.
After the introduction of caffeic acid, the average particle size and Zeta potential of the casein-caffeic acid covalent complex nanoparticles (CCP) were 171.11 nm and - 37.73 mV, respectively. The three-phase contact angle was also increased to 89.8°. By using CCP to stabilize Pickering emulsion (CCE), the retention quantity of the embedded curcumin increased by 2.19-fold after 28 days. In the simulated gastric digestion, curcumin degradation in CCE was reduced by 61.84%, released slowly in the intestinal environment, and the final bioaccessibility was increased by 1.90-fold. In C. elegans, CCE significantly reduced ROS accumulation, increased SOD activity by 2.01-fold and CAT activity by 2.30-fold, decreased MDA content by 36.76%, prolonging the lifespan of nematodes by 13.33% under H O stimulation and improving bioavailability in vivo.
The results indictae that CCP-stabilized Pickering emulsion can efficiently implement the physiological activities of bioactive compounds in vitro digestion and C. elegans, and thus it can be regarded as a reliable delivery system for food and medicine. © 2023 Society of Chemical Industry.
近年来，食品级皮克林乳液递送系统的设计已成为提高生物活性物质低生物利用度的有效策略。基于蛋白质的皮克林乳液因其高生物相容性和负载能力而受到广泛关注。活性物质的生物利用度主要通过模拟体外胃肠道消化来评估。秀丽隐杆线虫作为抗氧化和抗衰老的模式生物，可以为活性物质的体内利用提供额外的生物学信息。
引入咖啡酸后，酪蛋白 - 咖啡酸共价复合纳米颗粒（CCP）的平均粒径和Zeta电位分别为171.11 nm和 - 37.73 mV。三相接触角也增加到89.8°。通过使用CCP稳定皮克林乳液（CCE），包埋的姜黄素在28天后的保留量增加了2.19倍。在模拟胃消化中，CCE中姜黄素降解减少了61.84% 在肠道环境中缓慢释放，最终生物可及性提高了1.90倍。在秀丽隐杆线虫中，CCE显著减少ROS积累，超氧化物歧化酶（SOD）活性增加2.01倍，过氧化氢酶（CAT）活性增加2.30倍，丙二醛（MDA）含量降低36.76%，在H₂O₂刺激下使线虫寿命延长13.33% 并提高体内生物利用度。
结果表明，CCP稳定的皮克林乳液可以在体外消化和秀丽隐杆线虫中有效实现生物活性化合物的生理活性，因此可被视为食品和药物的可靠递送系统。© 2023化学工业协会。
