玻璃和 Hemotek 膜饲养系统在疟疾传播研究中的比较分析。
Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research.
机构信息
Department of Medical Microbiology, Radboud University Medical Center, Radboud Institute for Health Sciences, Nijmegen 6500 HB, The Netherlands.
Department of Medical Microbiology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen 6500 HB, The Netherlands.
出版信息
Trans R Soc Trop Med Hyg. 2023 Jun 2;117(6):476-478. doi: 10.1093/trstmh/trac135.
BACKGROUND
Glass membrane feeders are used in malaria research for artificial blood feeding. This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system.
METHODS
Hemotek feeders were compared with glass feeders by assessing mosquito feeding rate, imbibed blood meal volume and Plasmodium falciparum infection intensity on mosquito guts.
RESULTS
While mosquito feeding rate and blood meal volume were comparable between Hemotek and glass feeders, a loss in transmission was observed using the Hemotek feeder with a conventional collagen membrane. There was no difference in transmission between both feeders when Parafilm was used as the membrane.
CONCLUSIONS
Hemotek feeders with a Parafilm membrane can be used as an alternative feeding system for malaria transmission research.
背景
玻璃膜饲养器用于疟疾研究中的人工血液喂养。本研究调查了 Hemotek 膜饲养器作为标准化替代喂养系统的使用情况。
方法
通过评估蚊子的喂养率、吸食的血餐量和蚊子肠道中的疟原虫感染强度，将 Hemotek 饲养器与玻璃饲养器进行比较。
结果
虽然 Hemotek 和玻璃饲养器的蚊子喂养率和血餐量相当，但使用传统胶原蛋白膜的 Hemotek 饲养器时观察到传播损失。当使用 Parafilm 作为膜时，两种饲养器之间的传播没有差异。
结论
使用 Parafilm 膜的 Hemotek 饲养器可以作为疟疾传播研究的替代喂养系统。
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