Kulikov V I, Levachev S M
Biokhimiia. 1987 Jul;52(7):1154-61.
The incorporation of [5,6(n)-3H]prostaglandin A1 (PGA1) and [1-14C]oleic acid into membrane phospholipids of rat liver microsomes was studied. It was shown that PGA1 is incorporated into phospholipids in a much lesser degree than oleic acid. PGA1 is incorporated into phosphatidylethanolamine and, in a lesser degree, into phosphatidylcholine and phosphatidylinositol + phosphatidylserine. The exogenous cofactors of fatty acid acylation (ATP, CoA, Mg2+) exert no marked influence on the incorporation of PGA1 into the phospholipids. PGA1 interacts with isolated rat liver phospholipids; the PGA1-phospholipid conjugate formed is not destroyed in the course of one- or two-dimensional thin-layer chromatography. On the other hand, PGA1 binding to unsaturated phosphatidylcholines is strictly dependent on the phospholipid oxidation index. It is concluded that PGA1 incorporation into rat liver phospholipids is a result of interaction of PGA1 with peroxidized phospholipids.
研究了[5,6(n)-3H]前列腺素A1（PGA1）和[1-14C]油酸掺入大鼠肝脏微粒体膜磷脂的情况。结果表明，PGA1掺入磷脂的程度远低于油酸。PGA1掺入磷脂酰乙醇胺，程度较低地掺入磷脂酰胆碱和磷脂酰肌醇+磷脂酰丝氨酸。脂肪酸酰化的外源性辅因子（ATP、辅酶A、Mg2+）对PGA1掺入磷脂没有显著影响。PGA1与分离的大鼠肝脏磷脂相互作用；形成的PGA1-磷脂缀合物在一维或二维薄层色谱过程中不会被破坏。另一方面，PGA1与不饱和磷脂酰胆碱的结合严格依赖于磷脂氧化指数。得出的结论是，PGA1掺入大鼠肝脏磷脂是PGA1与过氧化磷脂相互作用的结果。
