Van Dyke R A, Wood C L
Drug Metab Dispos. 1975 Jan-Feb;3(1):51-7.
Incubation of rat liver microsomal suspensions with [14C] halothane resulted in the binding of a metabolite of halothane to microsomal phospholipids and, to a lesser extent, to microsomal proteins. The binding was inhibited by carbon monoxide, required NADPH, and was greatly enhanced under anaerobic conditions. The binding to lipids occurred only to phospholipids, particularly those with characteristics of phosphatidylcholine and phosphatidylethanolamine. The binding was dependent on the time of incubation as well as on the amount of microsomes present.
用[14C]氟烷孵育大鼠肝微粒体悬浮液，导致氟烷的一种代谢产物与微粒体磷脂结合，在较小程度上也与微粒体蛋白结合。这种结合受到一氧化碳的抑制，需要NADPH，并且在厌氧条件下大大增强。与脂质的结合仅发生在磷脂上，特别是具有磷脂酰胆碱和磷脂酰乙醇胺特征的那些磷脂。这种结合取决于孵育时间以及微粒体的存在量。
