Department of Zoology, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada; Graduate Program in Cell & Developmental Biology, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada; The Life Sciences Institute, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Department of Zoology, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada; Graduate Program in Cell & Developmental Biology, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada; The Life Sciences Institute, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada; Djavad Mowafaghian Centre for Brain Health, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
STAR Protoc. 2023 Mar 17;4(1):102028. doi: 10.1016/j.xpro.2022.102028. Epub 2023 Jan 13.
The auxin-inducible degron (AID) provides reversible, spatiotemporal control for the knockdown of target proteins. Here, we present a protocol for AID-mediated protein knockdown in Caenorhabditis elegans. We describe steps for generating the knock-in mutants using two CRISPR-Cas9 genome editing techniques and preparing the auxin-containing nematode growth media (NGM) plates. We also detail AID-mediated spatiotemporal protein knockdown. For complete details on the use and execution of this protocol, please refer to Kurashina et al. (2021)..
生长素诱导的降解结构域（AID）为靶蛋白的敲低提供了可逆的、时空控制。在这里，我们提供了一个在秀丽隐杆线虫中进行 AID 介导的蛋白敲低的方案。我们描述了使用两种 CRISPR-Cas9 基因组编辑技术生成敲入突变体的步骤，以及准备含有生长素的线虫生长培养基（NGM）平板。我们还详细介绍了 AID 介导的时空蛋白敲低。有关此方案的使用和执行的完整详细信息，请参阅 Kurashina 等人。（2021）。
