Xu Huifang, Tang Xuxia, Chen Jingjing, Shi Ya, Liu Jun, Han Cheng, Zhu Xiaoyu, Zhang Tao, Zhou Jinghe, Miao Wenyu
Otolaryngology Department, Zhejiang Provincial Hospital of Traditional Chinese Medical, Hangzhou, China.
Hunter Biotechnology, Hangzhou, China.
J Neurosci Methods. 2023 Feb 15;386:109793. doi: 10.1016/j.jneumeth.2023.109793. Epub 2023 Jan 11.
Habituation learning is a simple and conserved behavior in all organisms which could be induced by repeated stimuli. However, no standard and universal methods for training and evaluating the habituation learning behavior in larval zebrafish were available.
This study aims to establish effective training and detection protocols for habituation learning behavior in larval zebrafish by using the ViewPoint system. For this purpose, the detection threshold of velocity-a parameter for distinguishing the escape reaction and the spontaneous motion, the detection sensitivity-a parameter for determining the size of the identified object, the number of stimuli, and the age of larvae were optimized to obtain the best performance.
In this study, the optimized parameters were as follows: the detection threshold of velocity at 13, the luminous intensity at 8 %, the detection sensitivity at 32, the number of stimuli at 150, and the age of larvae at 6 dpf. Furthermore, we validated the utility of the established protocol by showing a consistent memory impairment induced by cycloheximide (CHX).
A similar method was reported previously. However, the equipment used in those assays, including the hardware and software, were neither standard nor universal, which might impede the extensive application of the habituation learning assays. Here, we developed an alternative method for studying the habituation learning behavior in larval zebrafish using the ViewPoint system.
Our study provided an alternative method for studying the habituation learning behavior in larval zebrafish.
习惯化学习是所有生物体中一种简单且保守的行为，可由重复刺激诱导产生。然而，目前尚无用于训练和评估斑马鱼幼体习惯化学习行为的标准通用方法。
本研究旨在通过使用ViewPoint系统，为斑马鱼幼体的习惯化学习行为建立有效的训练和检测方案。为此，对速度检测阈值（用于区分逃避反应和自发运动的参数）、检测灵敏度（用于确定识别物体大小的参数）、刺激次数和幼体年龄进行了优化，以获得最佳性能。
在本研究中，优化后的参数如下：速度检测阈值为13，发光强度为8%，检测灵敏度为32，刺激次数为150，幼体年龄为6天胚胎期（dpf）。此外，我们通过显示环己酰亚胺（CHX）诱导的一致记忆损伤，验证了所建立方案的实用性。
此前曾报道过类似方法。然而，那些实验中使用的设备，包括硬件和软件，既不标准也不通用，这可能会阻碍习惯化学习实验的广泛应用。在此，我们开发了一种使用ViewPoint系统研究斑马鱼幼体习惯化学习行为的替代方法。
我们的研究为研究斑马鱼幼体的习惯化学习行为提供了一种替代方法。
