Department of Hepatic-Biliary-Pancreatic Surgery, The Affiliate Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China.
Oxid Med Cell Longev. 2023 Jan 4;2023:1728463. doi: 10.1155/2023/1728463. eCollection 2023.
RING finger protein-7 (RNF7) functions as a positive regulator in the progression of multiple malignancies. However, the underlying mechanism by which RNF7 contributes to pancreatic cancer (PC) is lacking. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to test the level of RNF7expression in PC cell lines and tissues. The role of RNF7 in PC tumorigenesis was analyzed by Cell Counting Kit-8 (CCK-8). 5-Ethynyl-20-deoxyuridine (EdU), wound-healing/Transwell assays, as well as a subcutaneous tumorigenesis model were constructed to assess the role of RNF7 in PC cells. The association between RNF7 and PI3K/Akt signaling were assessed by western blot and further confirmed by rescue experiments. The PC patients with upregulated expression of RNF7 had poor survival. Overexpression of RNF7 significantly facilitated PC proliferative and migrative and invasive properties in vitro and vivo; however, knockdown of RNF7exhibited the opposite results. Mechanistically, RNF7 promoted PANC-1 and SW1990 cell growth through impacting the activation of the PI3K/Akt signaling pathway. Our data demonstrated that RNF7 promoted PC tumorigenesis via activating the PI3K/Akt signaling pathway and might be regarded as one of the potential therapies to PC.
环指蛋白 7（RNF7）作为一种正调控因子，在多种恶性肿瘤的进展中发挥作用。然而，RNF7 促进胰腺癌（PC）的潜在机制尚不清楚。实时定量聚合酶链反应（qRT-PCR）用于检测 PC 细胞系和组织中 RNF7 的表达水平。通过细胞计数试剂盒-8（CCK-8）分析 RNF7 在 PC 肿瘤发生中的作用。构建 5-乙炔基-20-脱氧尿苷（EdU）、划痕/Transwell 测定以及皮下肿瘤发生模型，以评估 RNF7 在 PC 细胞中的作用。通过 Western blot 评估 RNF7 与 PI3K/Akt 信号通路的关联，并通过挽救实验进一步证实。RNF7 表达上调的 PC 患者生存不良。RNF7 的过表达显著促进了 PC 在体外和体内的增殖、迁移和侵袭特性；然而，RNF7 的敲低则表现出相反的结果。机制上，RNF7 通过影响 PI3K/Akt 信号通路的激活促进 PANC-1 和 SW1990 细胞的生长。我们的数据表明，RNF7 通过激活 PI3K/Akt 信号通路促进 PC 肿瘤发生，可能被视为治疗 PC 的潜在疗法之一。
