Biological Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar, Gujarat, 382355, India.
Chembiochem. 2023 Apr 3;24(7):e202200634. doi: 10.1002/cbic.202200634. Epub 2023 Feb 28.
DNA nanocages have been explored for abilities to influence cellular behavior and functions. Recent times have seen the development of new emergent functionalities of DNA nanodevices as a class of biomaterials with an immense capacity to interface with biological systems and with vast potential in disease diagnosis and therapeutics. Being chemically robust and biocompatible in nature, DNA nanocages have been surface modified and structurally fine-tuned to find emerging applications in the field of stem-cell therapy and tissue regeneration. DNA nanocages can be used for therapeutic angiogenesis that involves the induction of blood vessel formation and can be used to treat ischemic diseases like stroke or heart failure. This work addresses the effect of DNA nanocages' structural topology on their capacity to stimulate endothelial cell angiogenesis. We tested a panel of four DNA nanocage geometries and checked their potential on the differentiation of human umbilical vein endothelial cells (HUVECs). While different DNA nanocage geometries showed successful induction of angiogenesis and cell migration in HUVECs, tetrahedral DNA cages showed the maximum uptake and angiogenesis potential, thus indicating that not only the composition of materials, but also the 3D arrangement of ligands might play role in stimulating angiogenesis.
DNA 纳米笼因其能够影响细胞行为和功能的能力而被广泛研究。近年来，随着 DNA 纳米器件作为一类具有与生物系统接口的巨大能力和在疾病诊断和治疗方面的巨大潜力的生物材料的新出现的功能得到了发展。由于其化学稳定性和生物相容性，DNA 纳米笼已被表面修饰和结构微调，以在干细胞治疗和组织再生领域找到新的应用。DNA 纳米笼可用于治疗性血管生成，包括诱导血管形成，并可用于治疗中风或心力衰竭等缺血性疾病。这项工作研究了 DNA 纳米笼结构拓扑对其刺激内皮细胞血管生成能力的影响。我们测试了一组四种 DNA 纳米笼几何形状，并检查了它们在人脐静脉内皮细胞 (HUVEC) 分化中的潜力。虽然不同的 DNA 纳米笼几何形状在 HUVEC 中成功地诱导了血管生成和细胞迁移，但四面体 DNA 笼显示出最大的摄取和血管生成潜力，这表明不仅材料的组成，而且配体的 3D 排列也可能在刺激血管生成中发挥作用。
