银屑病间充质干细胞在体外刺激人脐静脉内皮细胞的血管生成。

Psoriatic mesenchymal stem cells stimulate the angiogenesis of human umbilical vein endothelial cells in vitro.

机构信息

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, No. 5 Dong San Dao Xiang, Jiefang Road, Taiyuan 030009, China.

出版信息

Microvasc Res. 2021 Jul;136:104151. doi: 10.1016/j.mvr.2021.104151. Epub 2021 Mar 1.

DOI:10.1016/j.mvr.2021.104151

PMID:33662409

Abstract

OBJECTIVE

To investigate the regulation of psoriatic dermal mesenchymal stem cells (p-DMSCs) in the expression of vascular growth factor (VEGF), and migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro.

METHODS

A co-culture model of HUVECs and dermal mesenchymal stem cells (DMSCs)was used in this study. After 7-day co-culture, changes in expression levels of VEGF mRNA and protein in HUVECs were assessed using RT-PCR and Western Blotting, respectively. Migration and tubular formation of HUVECs were also assessed following co-culture of DMSCs and HUVECs.

RESULTS

In comparison to either HUVECs alone or co-culture of n-DMSCs and HUVECs, co-culture of HUVECs and p-DMSCs significantly increased expression levels of both VEGF mRNA (p < 0.01 vs. HUVECs alone) and protein in HUVECs (p < 0.001 vs. both HUVECs alone and HUVECs co-cultured with n-DMSCs). Moreover, p-DMSCs stimulated HUVEC migration and vascular formation (p < 0.05 vs. both HUVECs alone and co-culture of n-DMSCs and HUVECs).

CONCLUSION

Psoriatic DMSCs can upregulate VEGF expression, and stimulate migration and angiogenesis of HUVECs, suggesting a pathogenic role of p-DMSCs in psoriasis.

摘要

目的

研究银屑病真皮间充质干细胞（p-DMSCs）对血管生长因子（VEGF）表达的调控，以及体外人脐静脉内皮细胞（HUVECs）的迁移和血管生成。

方法

本研究采用 HUVECs 和真皮间充质干细胞（DMSCs）共培养模型。共培养 7 天后，分别采用 RT-PCR 和 Western Blotting 检测 HUVECs 中 VEGF mRNA 和蛋白的表达水平。共培养 DMSCs 和 HUVECs 后，评估 HUVECs 的迁移和管状形成。

结果

与 HUVECs 单独培养或 n-DMSCs 与 HUVECs 共培养相比，HUVECs 和 p-DMSCs 共培养显著增加了 HUVECs 中 VEGF mRNA 的表达水平（p<0.01 与 HUVECs 单独培养相比）和蛋白（p<0.001 与 HUVECs 单独培养和 n-DMSCs 与 HUVECs 共培养相比）。此外，p-DMSCs 刺激 HUVEC 迁移和血管形成（p<0.05 与 HUVECs 单独培养和 n-DMSCs 与 HUVECs 共培养相比）。