Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, China.
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang, China.
Plant Signal Behav. 2023 Dec 31;18(1):2163342. doi: 10.1080/15592324.2022.2163342.
A nitrate transporter gene, named , from salt-tolerant L. B46 has been cloned. B46NRT2.1 contained the same domain belonging to the major facilitator superfamily (PLN00028). The results of the phylogenetic tree indicated that B46NRT2.1 exhibits sequence similarity and the closest relationship with those known nitrate transporters of the NRT2 family. Through RT-qPCR, we found that the expression of mainly happens in the root and leaf. Moreover, the treatment with NaCl, NaCO, and NaHCO could significantly increase the expression of . B46NRT2.1 was located in the plasma membrane. Through the study of yeast and plant salt response brought by overexpression, we have preliminary knowledge that the expression of makes yeast and plants respond to salt shock. There are 10 different kinds of cis-acting regulatory elements (CRES) in the promotor sequences of gene using the PlantCARE web server to analyze. It mainly includes hormone response, abscisic acid, salicylic acid, gibberellin, methyl jasmonate, and auxin. The gene's co-expression network showed that it was co-expressed with a number of other genes in several biological pathways, including regulation of NO long-distance transit, modulation of nitrate sensing and metabolism, nitrate assimilation, and transduction of Jasmonic acid-independent wound signal. The results of this work should serve as a good scientific foundation for further research on the functions of the gene family in plants (inbred line B46), and this research adds to our understanding of the molecular mechanisms under salt tolerance.
从耐盐的 L. B46 中克隆到一个硝酸盐转运体基因，命名为 。B46NRT2.1 含有属于主要易化剂超家族（PLN00028）的相同结构域。系统发育树的结果表明，B46NRT2.1 与已知的硝酸盐转运体 NRT2 家族具有序列相似性和最密切的关系。通过 RT-qPCR，我们发现 主要在根和叶中表达。此外，NaCl、NaCO 和 NaHCO 的处理可以显著增加 的表达。B46NRT2.1 位于质膜上。通过对酵母和植物盐响应的研究，我们初步了解到 的表达使酵母和植物对盐冲击产生响应。使用 PlantCARE 网络服务器分析 基因启动子序列中的 10 种不同的顺式作用调控元件（CRES）。它主要包括激素反应、脱落酸、水杨酸、赤霉素、茉莉酸甲酯和生长素。 基因的共表达网络表明，它与其他一些基因在几个生物途径中共同表达，包括调节 NO 远距离转运、调节硝酸盐感应和代谢、硝酸盐同化和茉莉酸非依赖性伤口信号转导。这项工作的结果应该为进一步研究 基因家族在植物（自交系 B46）中的功能提供良好的科学基础，并为我们理解耐盐性下的分子机制增添了新的内容。
