Laboratory of Cyanobacterial Systematics and Stress Biology, Department of Botany, Banaras Hindu University, Varanasi, India.
Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, Varanasi, India.
Res Microbiol. 2023 May;174(4):104027. doi: 10.1016/j.resmic.2023.104027. Epub 2023 Jan 14.
A moderately thermophilic, gram-positive genomospecies Anoxybacillus rupiensis TPH1 was isolated from Tatapani hot spring, Chhattisgarh, India. Genome of 3.70 Mb with 42.3% GC subsumed 4131 CDSs, 65 tRNA, 5 rRNA, 35 AMR and 19 drug target genes. Further, comparative genomics of 19 Anoxybacillus spp. exhibited an open pan genome of 13102 genes along with core (10.62%), unique (43.5%) and accessory (45.9%) genes. Moreover, phylogenomic tree displayed clustering of Anoxybacillus spp. into two distinct clades where clade A species harbored larger genomes, more unique genes, CDS and hypothetical proteins than clade B species. Further, distribution of azoreductases showed FMN-binding NADPH azoreductase (AzoRed1) presence in clade A species only and FMN-binding NADH azoreductase (AzoRed2) harboring by species of both clades. Heavy metal resistance genes distribution showed omnipresence of znuA, copZ and arsC in both clades, dispersed presence of cbiM, czcD, merA and feoB over both clades and harboring of nikA and acr3 by few species of clade A only. Additionally, molecular docking of AzoRed1, AzoRed2, ZnuA, CopZ, Acr3, CbiM, CzcD, MerA and NikA with their respective ligands indicated high affinity and stable binding. Conclusively, present study provided insight into gene repertoire of genus Anoxybacillus and a basis for the potential application of this thermophile in bioremediation of azo dyes and heavy metals.
从印度恰蒂斯加尔邦的塔塔帕尼温泉中分离到一株中等嗜热、革兰氏阳性的基因组种 Anoxybacillus rupiensis TPH1。基因组大小为 3.70Mb，GC 含量为 42.3%，包含 4131 个 CDS、65 个 tRNA、5 个 rRNA、35 个 AMR 和 19 个药物靶标基因。此外，对 19 种 Anoxybacillus spp. 的比较基因组分析显示，其具有一个开放的泛基因组，包含 13102 个基因，其中包括核心（10.62%）、独特（43.5%）和附属（45.9%）基因。此外，系统发育树显示，Anoxybacillus spp. 聚类为两个不同的分支，其中分支 A 物种的基因组较大，拥有更多的独特基因、CDS 和假设蛋白，而分支 B 物种则较少。此外，偶氮还原酶的分布显示，只有分支 A 物种中存在 FMN 结合 NADPH 偶氮还原酶（AzoRed1），而分支 A 和 B 物种都含有 FMN 结合 NADH 偶氮还原酶（AzoRed2）。重金属抗性基因的分布显示，znuA、copZ 和 arsC 在两个分支中普遍存在，cbiM、czcD、merA 和 feoB 在两个分支中分散存在，而 nikA 和 acr3 仅由分支 A 的少数物种携带。此外，AzoRed1、AzoRed2、ZnuA、CopZ、Acr3、CbiM、CzcD、MerA 和 NikA 与各自配体的分子对接表明，它们具有高亲和力和稳定的结合。综上所述，本研究深入了解了 Anoxybacillus 属的基因库，并为该嗜热菌在偶氮染料和重金属的生物修复中的潜在应用提供了依据。
