Lim Jia Chun, Goh Kian Mau, Shamsir Mohd Shahir, Ibrahim Zaharah, Chong Chun Shiong
Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
J Basic Microbiol. 2015 Apr;55(4):514-9. doi: 10.1002/jobm.201400621. Epub 2014 Dec 19.
The Anoxybacillus sp. SK 3-4, previously isolated from a hot spring, was screened for its heavy metals resistance (Al(3+), Mn(2+), Cu(2+), Co(2+), Zn(2+), and Ni(2+)) and the strain was found to be most resistant to aluminum. Significant growth of the strain was observed when it was grown in medium containing aluminum (200 mg L(-1)-800 mg L(-1)) with relative growth rates ranging between 77% and 100%. A gene encoding the aluminum resistance protein (accession number: WP_021095658.1) was found in genome of strain SK 3-4, which revealed high sequence identity (>95%) to its homologues from Anoxybacillus species. Sequence comparisons with two functionally characterized aluminum resistance proteins, namely G2alt and ALU1-P, showed 97% and 81% of sequence identity, respectively. Four putative metal binding sites were detected in SK 3-4 aluminum resistance protein and G2alt at same amino acid residue positions of 186, 195, 198, and 201. Strain SK 3-4 was found to be able to remove aluminum from aqueous solution. This study demonstrated that Anoxybacillus sp. SK 3-4 could be applied in the treatment of aluminum contaminated wastewater.
先前从温泉中分离出的嗜热栖热放线菌SK 3-4，对其重金属抗性（Al(3+)、Mn(2+)、Cu(2+)、Co(2+)、Zn(2+)和Ni(2+)）进行了筛选，发现该菌株对铝的抗性最强。当该菌株在含有铝（200 mg L(-1)-800 mg L(-1)）的培养基中生长时，观察到其显著生长，相对生长速率在77%至100%之间。在菌株SK 3-4的基因组中发现了一个编码铝抗性蛋白的基因（登录号：WP_021095658.1），该基因与其来自嗜热栖热放线菌属物种的同源物具有高度的序列同一性（>95%）。与两种功能已明确的铝抗性蛋白G2alt和ALU1-P的序列比较分别显示出97%和81%的序列同一性。在SK 3-4铝抗性蛋白和G2alt的186、195、198和201相同氨基酸残基位置检测到四个推定的金属结合位点。发现菌株SK 3-4能够从水溶液中去除铝。这项研究表明，嗜热栖热放线菌SK 3-4可应用于处理受铝污染的废水。
